反式肉桂醛对稻绿核菌细胞超微结构及3种呼吸酶活力的影响

为初步探究反式肉桂醛(TC)对稻绿核菌的抑菌机制，采用透射电子显微镜观察了TC处理后稻绿核菌菌丝细胞的超微结构，并测定了2、4、8、15及30 μg/mL系列质量浓度TC对稻绿核菌细胞壁完整性的影响，以及其对烟酰胺腺嘌呤二核苷酸-苹果酸脱氢酶(NAD-MDHase)、琥珀酸脱氢酶(SDHase)和三磷酸腺苷酶(ATPase)活力的影响。结果显示:经4 μg/mL的TC处理后，菌丝细胞内脂质体显著增多，线粒体结构变模糊。当TC质量浓度升高至30 μg/mL时，可破坏菌丝细胞壁的完整性，且对NAD-MDHase活力的相对抑制率为44.3%，对SDHase活力的相对抑制率为76.7%；而Na+-K+-ATPase、Ca2+-Mg2+-ATPase、Ca2+-ATPase及Mg2+-ATPase的活力随TC质量浓度升高呈U型变化，其中，4 μg/mL的TC对ATPase活力的抑制效果最强，相对抑制率达78.4%以上。研究表明，细胞壁及线粒体可能是TC抑制稻绿核菌菌丝生长的作用靶点，具有进一步研究的价值。

Effects of trans-cinnamaldehyde against Villosiclava virens on cell ultrastructure and activities of three respiratory enzymes

The antifungal mechanism of trans-cinnamaldehyde (TC) against Villosiclava virens was investigated. The alteration of cell ultrastructure was observed using transmission electron microscope. The integrity of cell wall and activities of nicotinamide adenine dinucleotide-malic dehydrogenase (NAD-MDHase), succinate dehydrogenase (SDHase) and adenosine triphosphatase (ATPase) of V. virens were determined after treated with TC at the mass concentration of 2, 4, 8, 15 and 30 μg/mL. The results showed that the contents of lipidosome increased significantly and the structure of mitochondrion was indiscernible after treatment with TC at the mass concentration of 4 μg/mL. With the mass concentration of TC increasing to 30 μg/mL, the integrity of cell wall in V. virens was destroyed and the relative inhibition of NAD-MDHase and SDHase were reduced by 44.3% and 76.7%, respectively. Differently, the activities of Na+-K+-ATPase, Ca2+-Mg2+-ATPase, Ca2+-ATPase and Mg2+-ATPase fluctuated as U-curve with the concentration increase of TC. When the mass concentration of TC was 4 μg/mL, the highest inhibitive activity of TC on ATPase was the achieved among all the tested concentrations and the relative inhibition of TC on ATPase was higher than 78.4%. These results suggested that the cell wall and mitochondrion were the possible action targets for TC to inhibit the mycelial growth.