燕麦镰孢菌侵染对不同抗性青稞叶片及根系生理指标的影响

以抗病青稞品种NQK-01-03和感病青稞品种甘青2号为材料,在室内盆栽条件下,采用苗期人工接种法来测定抗感青稞品种内部及品种之间受燕麦镰孢菌侵染后引起的植株叶片和根系Pro、可溶性糖、可溶性蛋白和MDA含量的动态变化规律。结果表明,NQK-01-03病叶Pro含量高于健叶,可溶性糖、可溶性蛋白和MDA含量低于健叶；病根Pro、可溶性蛋白和MDA含量高于健根,可溶性糖含量低于健根；甘青2号病叶和病根Pro、MDA含量高于健叶和健根,可溶性糖、可溶性蛋白含量低于健叶和健根。受燕麦镰孢菌侵染,感病青稞甘青2号和抗病青稞NQK-01-03之间病健叶和病健根生理变化结果表明,感病青稞甘青2号病叶Pro含量高于抗病青稞NQK-01-03,可溶性糖、可溶性蛋白和MDA含量低于抗病青稞NQK-01-03；而感病青稞甘青2号病根Pro、MDA含量高于抗病青稞NQK-01-03,可溶性糖、可溶性蛋白含量低于抗病青稞NQK-01-03。本试验得出,Pro含量高低与青稞品种抗病性呈负相关,而可溶性蛋白含量高低与品种抗病性成呈正相关。该结果为明确燕麦镰孢菌侵染对青稞叶片和根系生理机制提供理论依据。

Fusarium avenaceum Infection: Effects on Physiological Indexes of Leaves and Roots of Naked Barley with Different Resistance

In order to determine the effect of Fusarium avenaceum infection on naked barley leaves and roots physiological characteristics, Pro, soluble sugar, soluble protein and MDA contentand were tested in pot culture condition with an inoculation method. The results showed that the content of Pro in diseased leaves of resistant NQK-01-03 were higher than those in healthy ones, while soluble sugar, soluble protein and MDA in diseased leaves were lower than healthy ones. And the content of Pro, soluble protein and MDA in diseased roots of resistant NQK-01-03 were higher than those in healthy ones, while soluble sugar in diseased roots were lower than healthy ones. Meanwhile, the content of Pro and MDA in diseased leaves and roots of susceptible GanQing Ⅱ were higher than those in healthy ones, while soluble sugar and soluble protein in diseased leaves and roots were lower than healthy ones. At the same time, the content of Pro in diseased leaves of susceptible GanQing Ⅱ were higher than those in healthy ones of resistant NQK-01-03, while soluble sugar, soluble protein and MDA in diseased leaves of susceptible GanQing Ⅱ were lower than healthy ones of resistant NQK-01-03. And the content of Pro, MDA in diseased roots of susceptible GanQing Ⅱ were higher than those in healthy ones of resistant NQK-01-03, while soluble sugar and soluble protein in diseased roots of susceptible GanQing Ⅱ were lower than healthy ones of resistant NQK-01-03. There was a negative correlation between the content of Pro and resistance, and a positive correlation was found between soluble protein and resistance. This study provided theoretical basis on physiological mechanism of F. avenaceum infection naked barley leaves and roots.