Effects of Two Different Cooling Devices for Testicles Transport on Stallion Epididymal Sperm Quality |
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| Institution: | 1. AUB-Instituto Nazionale Fecondazione Artificiale, University of Bologna, Granarolo dell''Emilia, Bologna, Italy;2. Department of Veterinary Medical Sciences, University of Bologna, Ozzano Emilia, Bologna, Italy;3. Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, TX;1. Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY;;2. Department of Medicine, University of Heidelberg, Heidelberg, Germany;;3. Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA;;4. Department of Haematology, Churchill Hospital, Oxford University Hospitals NHS Foundation Trust, United Kingdom;;5. Duke University Medical Center, Durham, NC;;6. Novant Health, Charlotte, NC;;7. Department of Medicine, University of North Carolina School of Medicine, Chapel Hill, NC;;8. Department of Data Sciences, Dana-Farber Cancer Institute/Harvard School of Public Health, Boston, MA;;9. Division of Hematology/Oncology, Medical College of Wisconsin, Milwaukee, WI;;10. Southampton University Hospitals, Southampton, United Kingdom;;11. Leicester Royal Infirmary, Leicester, United Kingdom; and;12. Clatterbridge Cancer Centre, Liverpool, United Kingdom;1. Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech Republic;2. Department of Statistics, Faculty of Economics and Management, Czech University of Life Sciences, Prague, Czech Republic;1. Departamento de Biología, Comisión de Genética, Universidad Autónoma de Madrid (UAM), C. Darwin 2, E-28049 Madrid, Spain;2. Centro de Investigación en Biodiversidad y Cambio Global (CIBC-UAM), Universidad Autónoma de Madrid, C. Darwin 2, E-28049 Madrid, Spain;3. Centro Militar de Cría Caballar de Ávila (FESCCR- Ministerio de Defensa), 05005 Ávila, Spain;4. Centro de Investigación sobre la Desertificación, CIDE-CSIC, Valencia, Spain;5. Department of Animal Reproduction & Artificial Insemination, Veterinary Research Division, National Research Center, 12622 Giza, Egypt;1. Department of Zootechnic Sciences, Coimbra College of Agriculture, Polytechnic Institute of Coimbra, Bencanta, 3040-316 Coimbra, Portugal;2. CERNAS – Natural Resources, Environment and Society Centre-ESAC, Bencanta, 3040-316 Coimbra, Portugal;3. Universidade de Lisboa, Faculdade de Ciências, Centro de Biologia Animal, Campo Grande, 1749-016 Lisboa, Portugal;4. Associação Internacional de Criadores do Cavalo Ibérico de Tipo Primitivo-Sorraias, Barbacena, Portugal;5. Centro de Estudos em Ciência Animal (CECA), Instituto de Ciências Biomédicas Abel Salazar (ICBAS), University of Porto, Oporto, Portugal;1. Pferdezentrum Bad Saarow, Equine Reproduction Unit, Freie Universität Berlin, Bad Saarow, Germany;2. Clinic for Horses, Freie Universität Berlin, Berlin, Germany;1. Swedish University of Agricultural Sciences, Clinical Sciences, Uppsala, Sweden;2. Graf Lehndorff Institute for Equine Sciences, Vetmeduni Vienna, Austria and Neustadt (Dosse), Germany;3. Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, Vetmeduni Vienna, Vienna, Austria;4. Al-Nahrain University - High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Clinical Reproductive Physiology, Baghdad, Iraq |
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| Abstract: | This study evaluates the effects of two cooling devices and temperature for testicles storage on epididymal sperm quality after 24 hours; different levels of seminal plasma (0% and 10%) were evaluated on sperm after recovering. Testicles from six stallions were recovered immediately after castration (2) or at the slaughterhouse (4); of the same animal, one testicle was placed in Equitainer (+8°C), the other in a styrofoam box with ice (+3°C). After 24 hours, the temperature of parenchyma was measured, and testicles and epididymal were weighted. Sperm were flushed from the cauda epididymides with Kenney extender, total sperm number recorded and motility and viability evaluated immediately after flushing (T0) with or without 10% SP (G1 Eq 0%, G2 Eq 10%, G3 Ice 0%, G4 Ice 10%). Motility and viability were evaluated after 24 hours and 48 hours of storage at +4°C. Temperature of the parenchyma was lower in testicles stored in ice compared to Equitainer (3.2 ± 0.6°C and 8.6 ± 2.5°C, respectively; P < .05). Motility and viability at T0 were similar (P > .05) in G1 and G3, whereas addition of SP after recovery significantly improved motility only in samples stored in Equitainer (G2). Viability was higher (P < .05) in G2 than in G4. At T24 and T48, no differences (P > .05) in sperm quality were found between storage methods or samples with or without SP. In conclusion, equine testicles can be safely stored either at lower (+3°C) or higher (+8°C) temperature than +5°C. This can be useful, especially when testicles are shipped in a hot climate, where devices cannot guarantee optimal refrigeration conditions. |
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| Keywords: | Epididymal sperm Stallion Testicle Storage |
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