| 金叶女贞中熊果酸的提取工艺研究 |
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| 引用本文： | 陈亮,夏晓芬,侯忍,马玫瑰,梅芳芳,蔡力,周轩.金叶女贞中熊果酸的提取工艺研究[J].安徽农业科学,2011,39(30):18481-18483. |
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| 作者姓名： | 陈亮 夏晓芬 侯忍 马玫瑰 梅芳芳 蔡力 周轩 |
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| 作者单位： | 湖北师范学院生命科学学院,湖北黄石,435002 |
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| 基金项目： | 湖北省教育厅优秀中青年人才项目(Q20102501) |
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| 摘 要： | 目的]筛选金叶女贞中熊果酸的最佳提取工艺。方法]采用纤维素酶解法提取金叶女贞中熊果酸,以5%香草醛及高氯酸作为显色剂,通过正交试验确定显色的最佳条件,在波长550 nm处用分光光度法测定熊果酸的含量。结果]最佳显色条件为：0.3 ml浓度5%香草醛、1.0 ml高氯酸、温度60℃,此条件下女贞花中熊果酸提取率达2.037%。结论]正交试验表明,在最佳提取工艺条件下,熊果酸的提取率比较理想;提取试验表明,在金叶女贞不同器官中,果实中熊果酸含量最高。
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| 关 键 词： | 熊果酸 分光光度法 正交试验 金叶女贞（Ligustrum lucidum Ait.） |
Extraction Technology of Ursolic Acid in Ligustrum Vicaryi |
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| CHEN Liang et al.Extraction Technology of Ursolic Acid in Ligustrum Vicaryi[J].Journal of Anhui Agricultural Sciences,2011,39(30):18481-18483. |
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| Authors: | CHEN Liang |
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| Institution: | CHEN Liang et al(College of Life Sciences,Hubei Normal University,Huangshi,Hubei 435002) |
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| Abstract: | Objective] The aim of this research was to select the optimum extraction technology of ursolic acid in Ligustrum Vicaryi.Method] Taking 5% vanillin acetum and perchloric acid as the color reagents,ursolic acid was extracted from L.vicaryi by cellulose method.The optimum color condition was determined by orthogonal test;and the content of ursolic acid was determined at 550 nm.Conclusion] The optimum color condition was 0.3 ml 5% vanillin,1.0 ml perchloric acid under 60 ℃.Under this condition,the extraction rate of ursolic acid in the L.vicaryi flower was 2.037%.Conclusion] Results of orthogonal test showed that that the content of ursolic acid was the highest in the fruits of L.vicaryi. |
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| Keywords: | Ursolic acid Spectrophotometry Orthogonal test Ligustrum vicaryi |
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