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| 奶牛腹腔巨噬细胞的分离与鉴定 | |
| 引用本文： | 张剑,杨宏军,侯佩莉,张亮,丁家波,宋玲玲,何洪彬,马卫明.奶牛腹腔巨噬细胞的分离与鉴定[J].山东农业科学,2013,45(2):38-40. |
| 作者姓名： | 张剑 杨宏军 侯佩莉 张亮 丁家波 宋玲玲 何洪彬 马卫明 |
| 作者单位： | 1. 山东农业大学动物科技学院,山东泰安271018;山东省农业科学院奶牛研究中心,山东济南250131 2. 山东省农业科学院奶牛研究中心,山东济南,250131 3. 中国兽医药品监察所,北京,100081 4. 山东农业大学动物科技学院,山东泰安,271018 |
| 基金项目： | 公益性行业(农业)科研专项(200903027);现代农业(奶牛)产业技术体系岗位科学家专项 |
| 摘 要： | 以无血清的RPMI-1640培养液灌洗奶牛腹腔,分离获取奶牛腹腔巨噬细胞,在含10%小牛血清的RPMI-1640培养液中培养。采用倒置显微镜观察细胞形态,并通过瑞氏染色计算其纯度。结果表明,采用无血清的RPMI-1640培养液灌洗奶牛腹腔,能够获得较高纯度的巨噬细胞,能为奶牛胞内寄生菌存活机制的研究提供很好的试验材料。 |
| 关 键 词： | 奶牛 巨噬细胞 分离培养 |
Separation and Characterization of Dairy Cow Peritoneal Macrophages |
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| Zhang Jian,Yang HongJun,Hou PeiLi,Zhang Liang,Ding JiaBo,Song LingLing,He HongBin,Ma WeiMing.Separation and Characterization of Dairy Cow Peritoneal Macrophages[J].Shandong Agricultural Sciences,2013,45(2):38-40. | |
| Authors: | Zhang Jian Yang HongJun Hou PeiLi Zhang Liang Ding JiaBo Song LingLing He HongBin Ma WeiMing |
| Institution: | 1*(1.College of Animal Science and Veterinary Medicine,Shandong Agricultural University,Taian 271018,China; 2.Research Center of Dairy Cow,Shandong Academy of Agricultural Sciences,Jinan 250131,China; 3.China Institute of Veterinary Drug Control,Beijing 100081,China) |
| Abstract: | The peritoneal cavity of dairy cow was douched with RPMI-1640 culture medium without fetal calf serum(FCS),and the peritoneal macrophages were separated and cultured in RPMI-1640 medium with 10% FCS.And then the cell morphology was observed under inverted microscopy,and the purity of macrophages was analyzed by Wright's staining.The results showed that highly purified macrophages were obtained by this method,which could be prepared for researches on the mechanism of intracellular bacterium living in dairy cow. |
| Keywords: | Dairy cow Macrophage Separation and culture |
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