钙果4号欧李组织培养技术研究

以钙果4号欧李秋季生长的幼嫩上部枝和春季萌生的幼嫩基生枝为材料,利用正交试验设计等方法研究了欧李组织培养的最佳基本培养基和植物生长调节剂配比。结果表明,以春季萌生的幼嫩基生枝为外植体材料较好,以70%～75%酒精浸洗10～30s后用0.1%升汞浸洗6min消毒效果最好,适宜芽诱导的培养基是改良MS+NAA0.1mg/L+6-BA1.0mg/L+20g/L蔗糖+7g/L琼脂,最佳芽增殖培养基是改良MS+6-BA2.0mg/L+NAA0.02mg/L+GA30.2mg/L+20g/L蔗糖+7g/L琼脂,适宜生根培养基是1/2MS+IBA1.0mg/L+KT0.02mg/L+20g/L蔗糖+7g/L琼脂。

Studies on tissue culture of Gaiguo 4, a line of Cerasus humilis

Experiment was carried out using the upper tender stem in Autumn and the inceptive tender stem in Spring of Gaiguo 4 (a line of Cerasus humilis Bunge) as material, and utilizing the orthogonal test to obtained the optimal medium for its tissue culture. Result showed: the inceptive tender stem in spring was the best explant. The best method for disinfectant was 70% to 75% alcohol (washed for 10s to 30s) and 0.1% mercuric chloride (washed for 6 min). The optimal medium for inducing shoot was improved MS NAA 0.1mg/L 6-BA 1.0 mg/L 20 g/L sugar 7 g/L agar; the optimal medium for shoot generation was improved MS 6-BA 2.0 mg/L NAA 0.02 mg/L GA3 0.2 mg/L 20 g/L sugar 7 g/L agar;the optimal medium for rooting is 1/2MS IBA 1.0 mg/L KT 0.02 mg/L 20 g/L sugar 7 g/L agar.