| 间歇浸没式生物反应器在辰星草培养苗扩繁中的应用 |
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| 引用本文： | 廉美兰,朴炫春,王颖,赵长新,金花.间歇浸没式生物反应器在辰星草培养苗扩繁中的应用[J].园艺学报,2004,31(4):532-536. |
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| 作者姓名： | 廉美兰 朴炫春 王颖 赵长新 金花 |
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| 作者单位： | (延边大学农学院园艺系，龙井133400) |
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| 摘 要： | 以茎尖培养得到的辰星草(Limonium hybrid‘MistyBIue’)培养苗(带2叶片)为试材。培养基为MS BA 1．0mg／L 蔗糖30g／L，pH调节为5．8。固体培养和振荡培养使用250mL三角瓶，每瓶100mL培养基。生物反应器培养使用5L气球型反应器，2L培养基。完全浸没式生物反应器培养时，无“载桥”，培养苗在培养基中始终处于浸泡状态；间歇浸没式生物反应器培养时，离反应器底部15cm处架有“载桥”，培养基定时与‘‘载桥”上的苗接触，每
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| 关 键 词： | 辰星草 间歇浸没式反应器 组织培养 |
| 文章编号： | 0513-353X(2004)04-0532-01 |
Application of Ebb and Flood Bioreactor System in Multiplication of Statice |
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| Lian Meilan,Piao Xuanchun,Wang Ying,Zhao Changxin,Jin Hua.Application of Ebb and Flood Bioreactor System in Multiplication of Statice[J].Acta Horticulturae Sinica,2004,31(4):532-536. |
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| Authors: | Lian Meilan Piao Xuanchun Wang Ying Zhao Changxin Jin Hua |
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| Institution: | 1. Department of Geriatrics, The Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;
2. Department of Physiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;
3. Department of Anesthesiology, Tengzhou Hospital of Traditional Chinese Medicine, Tengzhou 277500, China |
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| Abstract: | AIM: To study the effect of adrenomedullin (ADM) on L-type calcium currents (ICa,L) and its signaling transduction process in guinea-pig ventricular myocytes. METHODS: By using whole-cell patch clamp technique, the effect of ADM(1-100 nmol·L-1) on ICa,L was investigated in enzymatically dispersed single guinea-pig ventricular myocyte. Furthermore, the possible signaling transduction process of ADM on ICa,L was investigated by observing the effects of ADM22-52 (a specific ADM-receptor antagonist,100 nmol·L-1)+ADM(100 nmol·L-1), H-89 (a specific protein kinase A inhibitor, 10 μmol·L-1) + ADM(100 nmol·L-1), PKC19-36 (a specific protein kinase C inhibitor, 10 μmol·L-1) + ADM(100 nmol·L-1) and PMA (a specific protein kinase C activator, 1 μmol·L-1) on ICa,L, respectively. RESULTS: ADM at the concentrations of 1-100 nmol·L-1 decreased ICa,L in a dose-dependent manner (P<0.05) and ADM22-52 completely abolished the ADM-induced inhibition of ICa,L. H-89 did not attenuate the effects of ADM on ICa,L. Intracellular application of PKC19-36 prevented ADM-induced inhibition of ICa,L, while PMA could mimic the effects of ADM on ICa,L. CONCLUSION: ADM inhibits ICa,L, in guinea-pig ventricular myocytes, which is mediated by the specific ADM-receptor and probably related to PKC pathway. |
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| Keywords: | Adrenomedullin Calcium channels L-type Myocardium Signal transduction |
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