水稻去泛素化酶OsUCH-L5基因的克隆及表达分析

通过生物信息学及RT-qPCR(quantitative Real-time PCR,RT-qPCR)的方法对水稻OsUCH-L5基因进行探究.生物信息学预测结果表明,该基因全长837 bp,编码278个氨基酸;OsUCH-L 5蛋白的相对分子质量为31.64 kDa,等电点为5.66,为亲水性蛋白,具备Peptida...

Cloning and Expression Analysis of OsUCH-L5 Gene Encoded Rice Deubiquitin Enzyme

In this study,the rice OsUCH-L5 gene was investigated based on bioinformatics and quantitative Real-time PCR(RT-qPCR).The results of bioinformatics showed that the total length of the gene was 837 bp,encoding 278 amino acids.The relative molecular weight of OsUCH-L 5 protein was 31.64 kDa,and the isoelectric point was 5.66.OsUCH-L 5 protein belonged to hydrophilic protein and possessed Peptidase_C 12 and UCH_C domains.The phylogenetic tree indicated that OsUCH-L 5 protein was the most closely related to Brachypodium distachyon UCH-L 5.RT-qPCR results showed that OsUCH-L5 gene was tissue-specific,especially significant expressed in roots.Under different drought treatments with abscisic acid(200μmol·L^-1),D-mannitol(1 mmol·L^-1)and sodium chloride(250 mmol·L^-1),the gene expression levels were significantly different,and the maximum expression levels were found at 0.5 h,6 h and 12 h,respectively.Under the treatment with 200μmol·L^-1brassinolide,the expression of OsUCH-L5 gene reached the maximum at 3 h,and the expression of OsUCH-L5 gene was also induced by the low temperature of 4℃and high temperature of 40℃,that was,the gene expression reached the maximum at 3 h and 1 h respectively.Therefore,this study provided a basis for further exploration of the role of UCH family in regulating rice resistance,signal transduction,nutrition and salt stress regulation.