miR-423-5p在牛肌肉组织中表达及其靶基因预测

miR-423-5p在细胞中具有重要的生物学功能,如在肌细胞中能影响细胞的增殖。根据miRBase及NCBI网站显示的相关信息利用分析软件对miR-423在各物种之间的同源性进行了分析。取成年西门塔尔牛体内的骨骼肌、小肠、心脏组织利用茎环荧光定量PCR进行miR-423-5p表达量检测,将牛骨骼肌卫星细胞(MDSCs)分化处理不同时长后进行检测;最后通过在线软件(TargetScan、PicTar)预测其可能结合的靶基因。结果表明,miR-423-5p在各物种间具有较高的同源性,在骨骼肌中表达量明显高于其他组织。在MDSCs中,随着细胞分化时间的延长,miR-423-5p表达量逐渐升高。miR-423-5p在12个基因mRNA的3'-UTR上含有潜在的结合位点,其中部分靶基因参与细胞的增殖、分化和凋亡。结果提示,miR-423-5p可能参与细胞的生长代谢,调节细胞的增殖与分化。

Expression of miR-423-5p in bovine muscle and predicted target genes

miR-423-5p has important biological functions in cells, for example, it can affect cell proliferation in myocytes. In this paper, homology analysis was performed based on the relevant information displayed on miRBase and NCBI websites using analysis software. Skeletal muscle, small intestine and heart tissues from adult Simmental cattle were taken for miR-423-5p expression assay using stem-loop real-time PCR, and the same assay was performed after differentiation of bovine skeletal muscle satellite cells (MDSCs) treated with different time. Finally, the possible binding target genes were predicted by online software (TargetScan, PicTar). The results showed that miR-423-5p had a high homology among species and significantly higher expressed in skeletal muscle than in other tissues. In MDSCs, miR-423-5p expression gradually increased as the differentiation time of cells increased. miR-423-5p contained potential binding sites on the 3'UTR of 12 gene mRNAs, some of the target genes were involved in cell proliferation, differentiation and apoptosis. The results suggested that miR-423-5p may be involved in cell growth metabolism and regulate cell proliferation and differentiation.