| DNA不同提取方法对养殖池塘底泥细菌多样性PCR-DGGE检测的影响 |
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| 引用本文： | 王宇,林文辉,王亚军,毕晔,石存斌,吴淑勤. DNA不同提取方法对养殖池塘底泥细菌多样性PCR-DGGE检测的影响[J]. 吉林农业大学学报, 2009, 31(6) |
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| 作者姓名： | 王宇 林文辉 王亚军 毕晔 石存斌 吴淑勤 |
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| 作者单位： | 中国水产科学院珠江水产研究所,广州,510380;大连水产学院生命科学与技术学院,大连,116023;中国水产科学院珠江水产研究所,广州,510380 |
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| 基金项目： | 国家科技支撑计划项目(2006BAD03B05);;广东省科技计划项目(2006B20301028) |
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| 摘 要： | 应用PCR-DGGE技术研究了养殖池塘底泥中细菌DNA 5种提取方法对细菌多样性检测的影响。结果显示:(1)DNA粗提液中腐殖酸与蛋白的纯度无正相关;(2)5种方法提取的DNA粗提液经过纯化后进行PCR均取得良好结果;(3)采用复合破壁方法提取养殖池塘底泥细菌DNA有利于显示细菌多样性;(4)DNA提取方法是影响池塘底泥细菌多样性PCR-DGGE结果的关键因素,与DNA的产量、纯度关系甚微;(5)5种方法相似性高达0.79,在对养殖池塘底泥细菌多样性进行粗略分析时均可使用;(6)本试验中,基于改进的Martin-Laurent法是用于养殖池塘底泥细菌多样性PCR-DGGE研究的较好的总DNA提取方法。
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| 关 键 词： | 养殖池塘底泥 细菌多样性 DNA提取 PCR-DGGE 优势种群 |
Influence of Different DNA Extraction Methods on Microbial Diversity PCR-DGGE Detection of Culture Pond Sendiment |
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| WANG Yu,LIN Wen-hui,WANG Ya-jun,BI Ye,SHI Cun-bin,WU Shu-qin. Influence of Different DNA Extraction Methods on Microbial Diversity PCR-DGGE Detection of Culture Pond Sendiment[J]. Journal of Jilin Agricultural University, 2009, 31(6) |
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| Authors: | WANG Yu LIN Wen-hui WANG Ya-jun BI Ye SHI Cun-bin WU Shu-qin |
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| Affiliation: | WANG Yu1,2,LIN Wen-hui1,WANG Ya-jun1,BI Ye1,SHI Cun-bin1,WU Shu-qin11.Research Institute of Pearl River Fisheries,Chinese Academy of Fishery Sciences,Guangzhou 510380,China,2.College of Aqua-life Science , Technology,Dalian Fisheries University,Dalian 116023 |
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| Abstract: | In this research,PCR-DGGE technique was used for studying five different extraction methods of microbial DNA of the pond sediment in difference of microbial diversities.The purpose is to try to find extraction methods of DNA which can be best used to objectively reflect the diversities and advantages of the bacteria of the pond sediment,and to evaluate comprehensively the five extraction methods.The results showed that: DNA in the crude extract of two humic acids and protein purity is of no positive correla... |
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| Keywords: | PCR-DGGE |
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