Screening global <Emphasis Type="Italic">Medicago</Emphasis> germplasm for weevil (<Emphasis Type="Italic">Hypera postica</Emphasis> Gyll.) tolerance and estimation of genetic variability using molecular markers |
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Authors: | Amaresh Chandra |
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Institution: | (1) Crop Improvement Division, Indian Grassland and Fodder Research Institute, Gwalior Road, Jhansi, Uttar Pradesh, 284003, India |
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Abstract: | The genus Medicago encompasses many important forage species for both temperate and tropical regions. M. sativa L., commonly known as lucerne, is one of the most important forage species grown worldwide, but its production suffers seriously
from weevil (Hypera postica Gyll.) infestation. The aim of this work was to identify species/accessions with tolerance to weevil and their molecular
analysis using simple sequence repeat (SSR) markers. After screening 197 global germplasm encompassing 50 Medicago species for weevil tolerance, 22 lines representing 13 species were identified where leaf damage was ≤15% (P ≤ 0.05). In total, 37 accessions of the 22 lines, five Indian lucerne cultivars with leaf damage ≥75% and 10 accessions of
the 13 Medicago species with low to high infestation (>25%) were molecularly assessed using 11 SSR markers (5 newly developed) to delineate
closest to lucerne lines for breeding. In total, 33 bands were scored. The SAHN clustering using UPGM algorithm resulted into
two main clusters supported with high boot strap values and with genetic similarity ranging from 0.33 to 0.96. Two accessions
of M. tenoreana were observed closest to Indian lucerne cultivars. The rich variability revealed can be used as potential resource for transferring
genes across species. Although the inter-specific hybridization is difficult preposition in genus Medicago largely due to post fertilization barrier, the identified species/accessions can be utilized on priority in breeding programs
especially employing biotechnological tools like culturing of fertilized pods, ovule-embryo culture and electroporation. |
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Keywords: | Alfalfa Insect infestation Lucerne SSR Tolerance |
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