| 荷叶黄酮类化合物提取工艺研究 |
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| 引用本文： | 邓国栋,庄大海.荷叶黄酮类化合物提取工艺研究[J].贵州大学学报(农业与生物科学版),2010,29(5):428-431. |
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| 作者姓名： | 邓国栋 庄大海 |
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| 作者单位： | 贵州大学生命科学学院,贵州贵阳550025 |
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| 基金项目： | 贵州省科学技术基金资助项目，贵州大学博士基金资助项目 |
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| 摘 要： | 以总黄酮得率为指标,利用单因素试验确定提取溶剂,通过正交试验法优化提取工艺参数。结果表明：以乙醇为溶剂,回流提取的最佳工艺条件为：提取温度70℃,乙醇体积分数45%,料液比1∶20,提取时间3 h,回流提取2次。在此条件下,总黄酮得率为8.8%,说明该提取工艺设计合理,稳定可行,操作简便,为荷叶黄酮的研究和开发提供了借鉴。
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| 关 键 词： | 荷叶 总黄酮 提取工艺 优化 |
The Extraction Procedure of Total Flavonoids from Lotus Leaf |
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| DENG Guo-dong,ZHUANG Da-hai.The Extraction Procedure of Total Flavonoids from Lotus Leaf[J].Journal of Mountain Agriculture & Biology,2010,29(5):428-431. |
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| Authors: | DENG Guo-dong ZHUANG Da-hai |
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| Institution: | (College of Life Sciences,Guizhou University,Guiyang Guizhou 550025,China) |
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| Abstract: | To optimize the extraction procedure of total flavonoids from lotus leaf,the optimal extraction solvents and extraction process were examined by single factor test and orthogonal test,respectively.The optimal parameters were 45% ethanol,1∶20 solid-liquid ratio,2 times of extraction at 70℃ and each 3h,by which the total flavonoids yield rate was 8.8%.The extraction procedure was reliable and simple,which provided a basis for further exploitation of natural resource. |
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| Keywords: | Lotus leaf Total flavonoids extraction process optimization |
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