Determination of bovine rotavirus G serotype by polymerase chain reaction |
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| Authors: | Anjan Mondal Ranjit Aich Sharmila Majee Anilkumar Sadashivrao Bannalikar |
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| Institution: | (1) Department of Microbiology, West Bengal University of Animal and Fishery Sciences, Belgachia, Kolkata, 700037, India;(2) West Bengal University of Animal and Fishery Sciences, Belgachia, Kolkata, 700037, India;(3) Bombay Veterinary College, Parel, Mumbai, 400012, India |
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| Abstract: | A total of 113 diarrheic samples comprising of 68 buffalo calves and 45 cow calves were screened by RNA-PAGE for the detection
of presence of rotavirus. RNA-PAGE analysis of these samples revealed 11 (9.73%) was found positive for rotavirus. Out of
68 faecal samples of buffalo calves tested for viral gastroenteritis, 8 (11.76%) were found positive for rotavirus. Similarly,
out of 45 faecal samples of cattle calves tested for viral gastroenteritis, 3 (6.66%) was found positive for rotavirus. Rotavirus-positive
samples represented long electropherotype. All RNA-PAGE-positive faecal samples for rotavirus subjected to RT-PCR for VP7
gene, ten samples yielded a specific product of 1,013 bp of VP7 gene. All the PCR-positive samples of the present study were
subjected to genotyping with primers for G6, G8 and G10 genotype. All positive samples showed G10 genotype. This indicates
that G10 may be predominant genotype among bovine calves in Mumbai region in India. |
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