| 抗猪瘟病毒单克隆抗体的研究——猪瘟病毒抗原的提纯 |
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| 引用本文： | 王承浩,周作红,王亚鸣,王兴金,沈秋姑,徐兰芳. 抗猪瘟病毒单克隆抗体的研究——猪瘟病毒抗原的提纯[J]. 江西农业大学学报, 1989, 0(1) |
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| 作者姓名： | 王承浩 周作红 王亚鸣 王兴金 沈秋姑 徐兰芳 |
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| 作者单位： | 江西农业大学畜牧兽医系(王承浩，周作红，王亚鸣，王兴金，沈秋姑)，江西农业大学畜牧兽医系(徐兰芳) |
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| 摘 要： | 本文介绍了用 PEG 和蔗糖密度梯度区带离心纯化猪瘟病毒的方法和步骤。先用 PEG 沉淀浓缩猪瘟兔化弱毒感染的牛睾丸细胞培养液,再经蔗糖密度梯度区带离心,出现4条区带,其中病毒感染力最强的位于Ⅱ带,其次为Ⅰ带,蛋白质含量多为0.14～0.19毫克/毫升和0.21～0.12毫克/毫升。电镜检查可见带有膜囊的病毒粒子,SDS—聚丙烯酰胺凝胶电泳显示Ⅰ、Ⅱ、Ⅲ、Ⅳ带均各出现一条明显蓝色条带外,在它前后侧还隐约可见一条浅带。用这些方法浓缩提纯兔化猪瘟弱毒效果较好,且较简便易行。
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| 关 键 词： | 提纯 中国株猪瘟兔化弱毒 蔗糖密度梯度区带离心 SDS—聚丙烯酰胺凝胶电泳 |
CONCENTRATION AND PURIFICATION OF HOG CHOLERA VIRUS CULTIVATED IN FETAL TESTIS CELLS |
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| Wang Chenghao et al. CONCENTRATION AND PURIFICATION OF HOG CHOLERA VIRUS CULTIVATED IN FETAL TESTIS CELLS[J]. Acta Agriculturae Universitis Jiangxiensis, 1989, 0(1) |
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| Authors: | Wang Chenghao et al |
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| Abstract: | The lapinized Chinese strain of hog cholera virus cultivated in fetal testis mo-nolayer culture was concentrated by means of polyethylene glycol(PEG)preci-pitation.The concentrated virus was then again purified by zonal centrifugation insucrose density gradient.Electron microscopic examination and SDS-PAGE of thefinal preparation revealed that the lapinized Chinese strain of hog cholera virusparticles had been highly purified. |
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| Keywords: | purification the lapinized chinese strain of hog Cholera Virus the zonal centrifugation in sucrose density gradient SDS-PAGE |
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