| 采用孢子萌发法测定化合物对黄瓜白粉病菌的生物活性 |
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| 引用本文： | 杨小军,;倪汉文;杨立军;王少南;喻大昭.采用孢子萌发法测定化合物对黄瓜白粉病菌的生物活性[J].植物保护,2007,33(1):75-77. |
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| 作者姓名： | 杨小军 ;倪汉文;杨立军;王少南;喻大昭 |
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| 作者单位： | 湖北省农业科学院植保土肥研究所，中国农业大学农学与生物技术学院，湖北省农业科学院植保土肥研究所，湖北省农业科学院植保土肥研究所，湖北省农业科学院植保土肥研究所 武汉430064，中国农业大学农学与生物技术学院，北京100094，北京100094，武汉430064，武汉430064，武汉430064 |
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| 基金项目： | 国家科技攻关计划;国家人事部资助留学回国人员项目 |
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| 摘 要： | 接种黄瓜白粉菌后16 h的黄瓜叶片用透明胶带在子叶表面粘孢子取样的平均发芽率与用台盼蓝染色后在光学显微镜下调查的发芽率分别为83.7%和85.0%,t测验无显著差异,表明透明胶带取样的发芽率可以代表黄瓜叶片上白粉病菌孢子的发芽率。采用孢子萌发法和叶碟法分别测定了醚菌酯、植物源活性组分大黄素甲醚(P3D)对8个黄瓜白粉菌菌株的EC50。统计结果表明:采用两种方法测定醚菌酯P、3D对黄瓜白粉菌的EC50的相关系数的平方值分别为0.880、.99,表明两种方法的测定结果有很强的相关性;采用该孢子萌发法可评价化合物对植物专性寄生病菌如白粉菌孢子萌发的生物活性。
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| 关 键 词： | 孢子萌发法 白粉病菌 生物活性 |
| 修稿时间： | 2006-03-01 |
A novel bioassay method-determining the bioactivity of chemical compounds to cucumber powdery mildew by spore germination |
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| Yang Xiaojun;;Ni Hanwen;Yang Lijun;Wang Shaonan;Yu Dazhao.A novel bioassay method-determining the bioactivity of chemical compounds to cucumber powdery mildew by spore germination[J].Plant Protection,2007,33(1):75-77. |
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| Authors: | Yang Xiaojun;;Ni Hanwen;Yang Lijun;Wang Shaonan;Yu Dazhao |
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| Institution: | 1. Institute for Plant Protection and Soil Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, China ; 2. Agronomy and Biotechnology College, China Agricultural University, Beijing 100094, China |
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| Abstract: | The reliability of a novel bioassay method that was designed to determine the bioactivity of chemical compounds to powdery mildew based on spore germination inhibition was studied.The results showed that sampling spore germination by transparent tapes was simple and reliable.There was a significant correlation between the results by spore germination method and that by leaf disc assay no matter whether synthetic fungicide kresoxim-methyl or biofungicide P3D was used on cucumber powdery mildew.The results indicated that the spore germination method could replace the leaf disc method in fungicide bioassay against this disease. |
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| Keywords: | spore germination method powdery mildew bioactivity |
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