| 萝卜诱导愈伤组织较适外植体及激素的筛选 |
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| 引用本文： | 龙雯虹,杨荣萍,王建飞,张应华.萝卜诱导愈伤组织较适外植体及激素的筛选[J].河南农业科学,2006(2):93-95. |
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| 作者姓名： | 龙雯虹 杨荣萍 王建飞 张应华 |
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| 作者单位： | 云南农业大学园林园艺学院,云南,昆明,650201 |
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| 摘 要： | 以萝卜无菌苗的子叶和下胚轴为试验材料,比较了不同浓度的6-BA、NAA、2,4-D和IBA组合时,子叶和下胚轴的愈伤组织诱导率和质量,结果表明:子叶诱导愈伤组织的效果比下胚轴诱导愈伤组织的效果好,子叶是萝卜组织培养的较佳外植体;子叶和下胚轴为外植体诱导愈伤组织的最适培养基一致,均为MS 3 mg/L 6-BA 0.5 mg/L NAA 3%蔗糖。
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| 关 键 词： | 萝卜 愈伤组织 植物激素 子叶 下胚轴 |
| 文章编号： | 1004-3268(2006)02-0093-03 |
| 收稿时间： | 2005-09-28 |
| 修稿时间： | 2005年9月28日 |
Optimal Explants and Hormones for Callus Induction of Radish |
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| LONG Wen-hong,YANG Rong-ping,WANG Jian-fei,ZHANG Ying-hua.Optimal Explants and Hormones for Callus Induction of Radish[J].Journal of Henan Agricultural Sciences,2006(2):93-95. |
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| Authors: | LONG Wen-hong YANG Rong-ping WANG Jian-fei ZHANG Ying-hua |
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| Institution: | The College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, China |
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| Abstract: | Sterile cotyledons and hypocotyls of radish were used as experiment materials in this experiment.The callus from both cotyledons and hypocotyls cultivated in MS mediums containing 6-BA,NAA,2,4-D and IBA were compared.The results showed that more and better callus was induced from cotyledons than from hypocotyls.Both cotyledons and hypocotyls shared the same optimal medium of MS 3mg/L 6-BA 0.5mg/L NAA 3% sucrose. |
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| Keywords: | Radish Callus Hormone Cotyledon Hypocotyl |
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