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嘧啶氧磷对松材线虫的有效杀线活性
引用本文:洪森,王新荣,徐汉虹.嘧啶氧磷对松材线虫的有效杀线活性[J].广东林业科技,2014,30(1):26-30.
作者姓名:洪森  王新荣  徐汉虹
作者单位:华南农业大学 天然农药与化学生物学教育部重点实验室,华南农业大学植物病理系,华南农业大学 天然农药与化学生物学教育部重点实验室
基金项目:国家自然科学(31000868)
摘    要:通过24孔板测定了几种农药原药和乙醇对松材线虫的杀线活性。试验结果表明嘧啶氧磷杀线活性最高,它的致死中浓度LC50为32.65μg/mL,95%的置信区间为25.08~42.51μg/mL;体积分数为5%的乙醇在20min内使94.4%的松材线虫麻痹,体积分数为10%的乙醇在90S内将96.7%的线虫杀死。另外,松材线虫浸入供试药剂的时间越长,所测定的LC50的值越小,克百威、灭线磷、嘧啶氧磷的LC50值从24h到72h分别由448.47,51.96,94.54μg/mL降低至51.47,36.94,32.65μg/mL。

关 键 词:松材线虫  嘧啶氧磷  乙醇  生物活性测定LC50
收稿时间:2013/9/26 0:00:00
修稿时间:2013/11/6 0:00:00

Potent Nematicidal Activity of Pirimioxyphos against Bursaphelenchus xylophilus
HONG Sen,WANG Xin-rong and XU Han-hong.Potent Nematicidal Activity of Pirimioxyphos against Bursaphelenchus xylophilus[J].Forestry Science and Technology of Guangdong Province,2014,30(1):26-30.
Authors:HONG Sen  WANG Xin-rong and XU Han-hong
Institution:Key Laboratory of Pesticide and Chemical Biology, South China Agricultural University
Abstract:Several pesticides and ethanol were tested against Bursaphelenchus xylophilus using 24-well plate in vitro. The results showed that the most active pesticide was pirimioxyphos with LC50 value of 32.65 μg/mL, and it's 95% confidence limit was 25.08--42.51 μg/mL; B. xylophilus were paralyzed within 20 minutes after they were immersed in the 5% ethanol, and were killed within 90 seconds after they were immersed in 10% ethanol. In addition, the longer the immersion time of the test agent against B. xylophilus, the smaller the LC50 value was, the LCs0 values of carbofuran, ethoprophos, pirimioxyphos reduced from 24 to 72 h by 448.47,51.96,94.54 μg/mL to 51.47,36.94,32.65 μg/mL.
Keywords:Bursaphelenchus xylophilus    pirimioxyphos  ethanol  bioassay  Lethal Concentration 50
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