新疆野生樱桃李(Prunus cerasifera)茎段与叶片培养及其植株再生

以新疆野生樱桃李为材料,研究了基本培养基、植物生长调节剂种类、浓度及配比、蔗糖浓度等多种因素对茎段腋芽萌发与生长、增殖以及叶片不定芽再生的影响,建立了其叶片再生体系。结果表明,1)植物生长调节剂组合IAA0.4mg/L+6-BA0.8mg/L较适合于樱桃李茎段腋芽的萌发与生长培养;2)正交试验筛选出不定芽的最佳增殖培养基为3/4MS+IAA0.5mg/L+6-BA1.0mg/L+蔗糖30g/L;3)ZT诱导不定芽再生的效果好于6-BA,ZT1.5mg/L+IBA0.05mg/L为诱导不定芽再生的最适植物生长调节剂组合;4)暗培养为叶片不定芽再生的必要条件,在最适不定芽再生的培养基上,暗培养14d的不定芽再生效果最好;5)樱桃李不定梢的最佳生根培养基为1/2MS+IBA0.4mg/L。

Tissue culture of wild myrobalan plum (Prunus cerasifera)stem segment,leaf explant and its plantlet regeneration

The wild myrobalan plum’s stem segments were used as material for studying the effects of basic media, plant growth regulators, sucrose concentration and other factors on the segment’s axillary bud’s germination, growth and multiplication in vitro. Results showed,(1) IAA 0.4 mg/L+ 6-BA 0.8 mg/L was optimal for germination and growth of the stem segment with axillary buds; (2) The best bud multiplying medium 3/4 MS medium + IAA 0.5 mg/L+ 6-BA 1.0 mg/L+ sugar 30 g/L; (3) ZT1.5 mg/L + IBA 0.05 mg/L was the optimal one for the regeneration of leaves, and ZT was better than 6-BA for plantlet regeneration; (4) The dark was necessary for the leaves to regenerate. After 14 days dark-culture the explants were transferred under light and began to directly regenerate adventitious buds; (5) The optimal rooting medium was 1/2 MS medium + IBA 0.4 mg/L.