| 黄颡鱼FZD家族4个基因的克隆、组织表达及对铜的响应 |
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| 引用本文： | 张丽晗,罗智,有文静,李丹丹,吴坤,潘亚雄.黄颡鱼FZD家族4个基因的克隆、组织表达及对铜的响应[J].水产学报,2018,42(5):625-632. |
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| 作者姓名： | 张丽晗 罗智 有文静 李丹丹 吴坤 潘亚雄 |
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| 作者单位： | 华中农业大学水产学院动物遗传育种与繁殖教育部重点实验室 |
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| 基金项目： | 国家自然科学基金（31422056） |
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| 摘 要： | 实验采用RT-PCR和RACE克隆fzd2、fzd3a、fzd4和fzd10基因,其c DNA全长分别为2 176、3 243、2 509和3 021 bp,其中ORF长度分别为1 652、2 084、1 649和2 161 bp,编码551、695、550和586个氨基酸。氨基酸序列比对和系统树分析显示,4种基因十分保守,黄颡鱼与斑点叉尾鲖亲缘关系最近。组织表达分析显示,4种基因的m RNA在脑、脾脏、肾脏、鳃、心脏、肌肉、脂肪、肝脏及卵巢等组织中都有表达,而其表达量不尽相同。FZD家族基因对铜的响应研究表明,在暴露28 d时,fzd3a m RNA水平随着铜浓度的增加而增加,但是fzd2、fzd4和fzd10基因表达各个处理组无显著性差异;在56 d,fzd2m RNA水平在30μg Cu/L组最高,其他2个组差异不显著,fzd3a、fzd4和fzd10的基因表达在3个铜暴露组中均无显著差异,表明fzd家族这些基因的功能发生了分化,部分成员介导了铜影响黄颡鱼卵巢发育的调控。
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| 关 键 词： | 黄颡鱼 FZD 分子特征 组织表达 铜 |
| 收稿时间： | 2017/4/18 0:00:00 |
| 修稿时间： | 2017/7/19 0:00:00 |
Molecular characterization and tissue distribution of Frizzled (FZD) in yellow catfish (Pelteobagrus fulvidraco) by copper exposure |
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| ZHANG Lihan,LUO Zhi,YOU Wenjing,LI Dandan,WU Kun and PAN Yaxiong.Molecular characterization and tissue distribution of Frizzled (FZD) in yellow catfish (Pelteobagrus fulvidraco) by copper exposure[J].Journal of Fisheries of China,2018,42(5):625-632. |
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| Authors: | ZHANG Lihan LUO Zhi YOU Wenjing LI Dandan WU Kun and PAN Yaxiong |
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| Institution: | Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China,Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China,Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China,Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China,Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China and Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China |
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| Abstract: | |
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| Keywords: | Pelteobagrus fulvidraco Frizzled (FZD) molecular characterization tissue distribution copper exposure |
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