Hemotropic mycoplasmas in bats captured near human settlements in Nigeria |
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| Institution: | 1. PhD Program in Conservation Medicine, Facultad de Ciencias de la Vida, Universidad Andres Bello, República 252, Santiago, Chile;2. Parasitology Division, National Veterinary Research Institute PMB 01 Vom, Plateau State, Nigeria;3. Facultad de Ciencias de la Vida, Universidad Andres Bello, República 252, Santiago, Chile;4. Instituto Agroalimentario de Aragón-IA2 (Universidad de Zaragoza-CITA), Miguel Servet 177, 50013, Zaragoza, Spain;5. Fundación ARAID, Avda. de Ranillas, 50018, Zaragoza, Spain;1. Pós-graduação em Microbiologia Agropecuária, Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil;2. Laboratório de Imunoparasitologia, Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias/Universidade Estadual Paulista (FCAV/UNESP), Jaboticabal, SP, Brazil;3. Laboratório de Biologia de Tripanosomatídeos, Fundação Oswaldo Cruz/FioCruz, Rio de Janeiro, RJ, Brazil;4. Direcção de Ciências Animais, Maputo, Moçambique;1. Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Romania;2. Department of Parasitology and Zoology, University of Veterinary Medicine, Budapest, Hungary;3. Section of Virology, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Box 7028, 750 07, Uppsala, Sweden;4. SLU Global Bioinformatics Centre, Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 7023, 750 07, Uppsala, Sweden;5. Consejo Nacional de Investigaciones Científicas y Técnicas, Godoy Cruz 2290, 1425, Ciudad Autónoma de Buenos Aires, Argentina;6. Instituto de Agrobiotecnología y Biología Molecular, IABiMo, INTA-CONICET, Calle Las Cabañas y Los Reseros s/n, Casilla de Correo 25, Castelar, 1712, Buenos Aires, Argentina;1. Laboratory of Immunoparasitology, Department of Veterinary Pathology, Universidade Estadual Paulista Júlio de Mesquita Filho, Jaboticabal, SP, Brazil;2. Department of Genetics and Animal Breeding, Universidade Estadual Paulista Júlio de Mesquita Filho, Jaboticabal, SP, Brazil;3. Department of Veterinary Clinical Pathology, Centro Universitário Moura Lacerda, Ribeirão Preto, SP, Brazil;1. Department of Biomedical Sciences, Ross University School of Veterinary Medicine, Saint Kitts and Nevis, West Indies, Cote d’Ivoire;2. Instituto de Ciencias Clínicas Veterinarias, Facultad de Ciencias Veterinarias, Universidad Austral de Chile, Valdivia, Chile;3. PhD Program in Conservation Medicine, Facultad de Ciencias de la Vida, Universidad Andres Bello, República 252, Santiago, Chile;4. Laboratorio de Ecología del Paisaje y Conservación, Departamento de Ciencias Forestales y Medioambiente, Universidad de La Frontera, P.D. Box 45-D, Temuco, Chile;5. Facultad de Ciencias de la Vida, Universidad Andres Bello, República 252, Santiago, Chile;6. Instituto Agroalimentario de Aragón-IA2 (Universidad de Zaragoza-CITA), Miguel Servet 177, 50013, Zaragoza, Spain;7. Fundación ARAID, Avda. de Ranillas, 50018, Zaragoza, Spain;8. Wildlife Ecology and Conservation Lab, Departamento de Zoología, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Casilla 160-C, Concepción, Chile |
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| Abstract: | The presence of DNA of hemotropic mycoplasmas (hemoplasmas) was investigated for the first time in bats in Africa. Blood samples from 90 bats captured within or near human settlements in nine study areas from five states in Nigeria belonging to six genera of the families Pteropodidae, Rhinolophidae, and Molossidae were analyzed using conventional PCR protocol targeting a 391 bp part of the 16S rRNA gene. Of these, 32 samples (35 %) resulted positive. Eight nucleotide sequence types were identified, which were assigned to five genotypes showing between 93–99 % similarity with hemoplasmas from bats and/or rodents from other parts of the world, and/or Candidatus Mycoplasma haemohominis from a human patient. Network analysis showed genetic structure of hemoplasma sequences among bat species, but some sequences were shared among bats of different taxonomic groups and distant study areas. Further characterization of the samples using a protocol targeting ～1200 bp of the 16S rRNA gene resulted in seven sequences that confirmed the results of the screening protocol. Hemoplasmas in Nigerian bats are prevalent, widely distributed and genetically diverse. The zoonotic risk to local inhabitants should not be neglected, due to the high similarity of some of the retrieved sequences with the human pathogen C. M. haemohominis. |
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| Keywords: | Africa Chiroptera Mollicutes Zoonosis |
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