| 离子色谱法测定寄生后小菜蛾血淋巴游离氨基酸 |
| |
| 引用本文： | 晁云飞,白素芬,何璠,李欣,黄晓书,杨改青,徐鹏.离子色谱法测定寄生后小菜蛾血淋巴游离氨基酸[J].河南农业大学学报,2009,43(3). |
| |
| 作者姓名： | 晁云飞 白素芬 何璠 李欣 黄晓书 杨改青 徐鹏 |
| |
| 作者单位： | 1. 河南农业大学植物保护学院,河南郑州,450002
2. 河南省高校农业生物技术与工程技术重点学科开放实验室,河南郑州,450002 |
| |
| 摘 要： | 采用离子色谱法测定了小菜蛾末龄幼虫、预蛹及被菜蛾盘绒茧蜂寄生后,血淋巴中游离氨基酸组成和含量.共检测到17种氨基酸,其中组氨酸、脯氨酸和丙氨酸是主要的种类.随着小菜蛾幼虫的生长,血淋巴中氨基酸总量明显增加,发育到预蛹时,总量下降,表明虫体内氨基酸代谢途径因变态的即将发生而产生了变化.被菜蛾盘绒茧蜂寄生后,小菜蛾幼虫血淋巴中游离氨基酸总量显著增加,导致多数氨基酸种类含量增加,表明该蜂有效地调节了寄主的蛋白质和氨基酸代谢,使之朝着有利于自身发育的方向发展.但是,当蜂幼虫完成在寄主体内的发育时,小菜蛾血淋巴中氨基酸总浓度明显下降,说明该蜂有效地获取了寄主中的氨基酸营养.对这些氨基酸种类的识别将可为今后该蜂大规模的的人工化培养提供极有价值的信息.
|
| 关 键 词： | 游离氧基酸 血淋巴 寄生 小菜蛾 菜蛾盘绒茧蜂 |
Determination of free amino acids in the hemolymph of parasitized Plutella xylostella by ion chromatography |
| |
| CHAO Yun-fei,BAI Su-fen,HE Fan,LI Xin,HUANG Xiao-shu,YANG Gai-qing,XU Peng.Determination of free amino acids in the hemolymph of parasitized Plutella xylostella by ion chromatography[J].Journal of Henan Agricultural University,2009,43(3). |
| |
| Authors: | CHAO Yun-fei BAI Su-fen HE Fan LI Xin HUANG Xiao-shu YANG Gai-qing XU Peng |
| |
| Institution: | 1.College of Plant Protection;Henan Agricultural University;Zhengzhou 450002;China;2.Key Laboratory of Biotechnology and Engineering of Henan Education Department;China |
| |
| Abstract: | Changes in free amino acids were analysed in hemolymph of fourth instar larvae,prepupae and parasitized Plutella xylostella larvae by ion chromatography.Seventeen primary amino acids were detected among which histidine,proline and alanine were the predominant free amino acids at all stages.The concentration of total amino acids increased significantly with the development of larvae.However,the amount declined with the larvae entering the prepupal phase.This suggested that changes in amino acid metabolism oc... |
| |
| Keywords: | free amino acid hemolymph parasitism Plutella xylostella Cotesia vestalis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
