产植物激素拮抗细菌CX-5-2的筛选、鉴定及其特性研究

 利用高效液相色谱筛选到能够同时产生IAA和GA₃的细菌菌株1株,产GA₃的细菌菌株2株。生测结果显示,菌株CX-5-2对小麦的促生长作用最明显,10倍稀释发酵液处理比清水对照处理可使麦苗高度增加10.54%。利用反相高效液相色谱法明确了菌株CX-5-2在不同培养条件下产生GA₃和IAA的积累情况;通过细菌16S rDNA的保守序列鉴定,菌株CX-5-2为假单胞菌属。本研究表明利用高效液相色谱可快速高效地筛选产激素类植物促生菌。

Selection, identification and characterization of antagonistic and phytohormone producing bacterial strain CX-5-2

The strains of phytohormones-producing bacteria were screened by using High Performance Liquid Chromatography(HPLC).Plant growth-promotion of these strains was determined on wheat seedlings.Strain CX-5-2 was selected and showed the most significant growth-promotion effect on wheat growth.The result showed that wheat seedlings irrigated with bacteria culture broth grew higher than that treated with water.The average height of wheat seedlings was increased about 10.54%.The accumulations of gibberellin and indole-3-acetic acid produced by CX-5-2 were measured with HPLC.Furthermore,CX-5-2 was also antagonistic to many pathogens through dual culture method.Identification of strain CX-5-2 was carried out with 16S rDNA ITS.The result indicated that strain CX-5-2 was belonged to genus Pseudomonas.HPLC was an efficient way to screen the strains of hormone producing bacteria.