红掌细菌性疫病的病原菌初步鉴定

 在云南西双版纳的红掌上发现一种由细菌侵染引起的病害,从叶片的病组织上分离到具有致病性的杆状细菌,将分离的病原菌接种于健康的红掌上,表现出与田间一致的症状。感病初期在叶缘或叶脉间出现水渍状小点,后期病斑多呈棕褐色至黑色坏死,病健交界处多呈黄色。从接种发病的病斑与田间标样上分离的病原菌菌落形态完全相同。通过菌株的培养性状、常规生理生化特性及透射电镜下菌体形态观察结果,将病原菌初步鉴定为黄单胞菌属(Xanthomonas)。BIOLOG鉴定和致病性测定结果进一步鉴定病原菌为地毯草黄单胞菌花叶万年青致病变种Xanthomonas axonopodis pv. dieffenbachiae(Xad)(McCulloch & Pirone) Vauterin et al.

Preliminary identification of bacterial strain causing Anthurium bacterial blight

In April 2002, a serious Anthurium disease broke out in a commercial plantation in Xishuang-banna , Yunnan Province, P. R. China. The early symptom appeared as irregular water-soaked spots on the margin or in the middle of leaves. Eventually, brown to blighted areas were often observed together with striking yellow zones along leaf margins. Yellow, mucoid colonies were consistently isolated from infected tissues. The strains reproduced the typical symptom after spraying or rubbing on both Anthurium and Dieffenbachia leaves. The colonies re-isolated from inoculated leaves were as same as those from infected tissues. The pathogen was preliminarily identified as genus Xanthomonas, based on bacterial morphological, cultural, physiological and biochemical properties. The pathogen was further identified as Xanthomonas axonopodis pv. dieffenbachiae (McCulloch and Pirone) Dye by carbon source utilization and pathogenicity tests.