湿地松松针中莽草酸的提取及含量测定

目的]为更好地开发利用湿地松资源提供科学依据。方法]研究了湿地松松针中莽草酸的提取工艺并用高效液相色谱测定了莽草酸含量。高效液相色谱的色谱条件：色谱柱：Alltima NH2（5μm,4.6mm×150.0mm）;移动相：乙腈-2%H3PO4（90∶10）;流速：1ml/min;测定波长为213nm,带宽为16nm;参比波长为300nm,带宽为80nm。结果]通过单因素试验和正交试验,确定乙醇浸提法提取最佳工艺：乙醇浓度为60%,提取温度为75℃,料液比为1∶25,提取时间为2.5h。在该条件下,莽草酸的提取率为1.49%。结论]湿地松松针可以作为新的莽草酸资源加以开发利用。

Extraction and Content Determination of Shikimic Acid in Pinus elliottii Engelm

Objective] The aim was to provide scientific basis for development and utilization of Pinus elliottii Engelm resources. Method] The extraction process of shikimic acid in Pinus elliottii Engelm was studied,and the content of shikimic acid was determined by HPLC. The HPLC conditions were as follows:Alltima NH2 (5 μm,4.6 mm × 150.0 mm) column separation;the mobile phase was Hexane-2%H3PO4(90∶10); the flowing velocity was 1 ml/min; test wavelength was 213 nm,the width of belt was 16 nm; compare wavelength was 300 nm,the width of belt was 80 nm. Result] By the single factor and orthogonal tests,the optimum conditions were found as follows:ethanol concentration 60%,extraction temperature 75 ℃,solid-liquid ratio 1∶25,extraction time 2.5 h.The extraction rate of shikimic acid was 1.49%. Conclusion] Shikimic acid in Pinus elliottii Engelm could be used as a new resource to develop and utilize.