Nucleotides in yeast tRNAPhe required for the specific recognition by its cognate synthetase |
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| Authors: | J R Sampson A B DiRenzo L S Behlen O C Uhlenbeck |
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| Affiliation: | Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309. |
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| Abstract: | ![]()
An analysis of the aminoacylation kinetics of unmodified yeast tRNAPhe mutants revealed that five single-stranded nucleotides are important for its recognition by yeast phenylalanyl-tRNA synthetase, provided they were positioned correctly in a properly folded tRNA structure. When four other tRNAs were changed to have these five nucleotides, they became near-normal substrates for the enzyme. |
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