马铃薯油菜素内酯信号激酶基因StBSKs的克隆与序列分析

油菜素内酯是一种参与调控植物生长发育和环境抗性的植物激素,BSKs是BR信号通路中重要的信号转导激酶。以马铃薯为试验材料,通过同源序列检索分析设计特异性引物,采用RT-PCR技术克隆得到BSK基因家族的7个基因,分别命名为StBSK1、StBSK2、StBSK3、StBSK4、StBSK5、StBSK6和StBSK7。其CDS全长分别为1 497、1 479、1 464、1 461、1 476、1 476和1 476 bp,分别编码498、492、487、486、491、491和491个氨基酸。生物信息学分析表明,StBSKs蛋白的等电点为5.14~6.37,均呈弱酸性;这7个蛋白均无跨膜结构域和信号肽;氨基酸序列比对发现,StBSKs蛋白的氨基酸序列在N端存在较大差异。系统进化树分析发现,StBSKs蛋白与同科物种的亲缘关系更近。本研究结果丰富了对马铃薯油菜素内酯信号激酶StBSKs基因的认知,也为进一步深入研究StBSKs的基因功能奠定基础。

Cloning and sequence analysis of StBSKs gene of Solanum tuberosum

Brassinolide (BR) is a kind of plant hormone that participates in the regulation of plant growth and environmental resistance. Brassinolide signal kinase (BSKs) is an important signal transduction kinase in BR signaling pathway. Solanum tuberosum used as material and specific primers were designed by homologous sequence retrieval. Seven genes of the StBSK gene family were cloned by RT-PCR technology, and respectively named StBSK1, StBSK2, StBSK3, StBSK4, StBSK5, StBSK6 and StBSK7. Total length of coding sequences were 1 497, 1 479, 1 464, 1 461, 1 476, 1 476 and 1 476 bp that encoded 498, 492, 487, 486, 491, 491 and 491 amino acid protein, respectively. Bioinformatics analysis showed that the isoelectric points of StBSKs protein were weakly acidic between 5.14 and 6.37, and these seven proteins had no transmembrane domain and signal peptide. Amino acid sequence alignment revealed that the amino acid sequences of StBSKs protein were different in N-terminal. Phylogenetic tree analysis showed that StBSKs protein was more closely related to the same species. The results of this study enriched the knowledge of the StBSKs gene and laid a foundation for further study on StBSKs gene function in Solanum tuberosum.