| Box-Behnken法优化大叶榉树黄酮类超声辅助提取方法及初步鉴定 |
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| 引用本文： | 刘晓玲,金晓玲,张旻桓,张亚平,曾艳,柴弋霞.Box-Behnken法优化大叶榉树黄酮类超声辅助提取方法及初步鉴定[J].浙江林业科技,2019(2):81-86. |
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| 作者姓名： | 刘晓玲 金晓玲 张旻桓 张亚平 曾艳 柴弋霞 |
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| 作者单位： | 中南林业科技大学风景园林学院;湖南省环境资源植物开发与利用工程技术研究中心;贵州农业职业学院 |
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| 基金项目： | 湖南省教育厅“十二五”重点学科资助项目(2011-76);国家林业局重点学科(林人发[2016]21号);中央财政林业科技推广项目([2015]XT008) |
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| 摘 要： | 为了探讨大叶榉树Zelkova schneideriana叶片黄酮类成分,以1年生大叶榉树叶片为材料,在超声波辅助下,通过单因素和Box-Behnken试验,探究并优化了大叶榉叶片黄酮类乙醇提取方法;采用常规显色法,对大叶榉树叶片中黄酮类物质种类进行初步鉴定。结果表明:(1)优化后的大叶榉树黄酮类提取方法为:乙醇浓度63%、液料比31:1mL·g^-1、提取时间1.7 h,大叶榉树黄酮类提取量为(31.067±0.11)mg·g^-1;(2)初步鉴定发现:大叶榉树叶片中含有黄酮、具有邻二酚羟基结构的黄酮类化合物、具有邻二苯酚羟基或兼有3-OH,4=O或5-OH,4=O的化合物;可能有黄酮醇、二氢黄酮、二氢黄酮醇等;不含查尔酮和橙酮。
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| 关 键 词： | Box-Behnken 响应面 交互作用 大叶榉树 黄酮类 |
Optimization of Ultrasonic Extraction of Flavonoids from Zelkova schneideriana Leaves by Box-Behnken |
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| LIU Xiao-ling,JIN Xiao-ling,ZHANG Min-huan,ZHANG Ya-ping,ZENG Yan,CHAIYi-xia.Optimization of Ultrasonic Extraction of Flavonoids from Zelkova schneideriana Leaves by Box-Behnken[J].Journal of Zhejiang Forestry Science and Technology,2019(2):81-86. |
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| Authors: | LIU Xiao-ling JIN Xiao-ling ZHANG Min-huan ZHANG Ya-ping ZENG Yan CHAIYi-xia |
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| Institution: | (College of Landscape Architecture,Central South University of Forestry and Technology,Changsha 410004,China;Hunan Research Center of Engineering Technology for Utilization of Environmental and Resource Plants,Changsha 410000,China;Guizhou Vocational College of Agriculture,Guiyang 551400,China) |
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| Abstract: | On August 26, 2017, collections were implemented on1-3 leaves of 1-year Zelkova schneideriana in greenhouse for experiment on extraction of flavonoids. Optimization of ultrasonic extraction of flavonoids was designed by box-behnken with three factors at three levels. The result demonstrated that the best technology for extraction was as follows, ethanol concentration of 63%, liquid ratio of 31:1 mL·g^-1, extraction time of 1.7 hour, with the yield of flavonoids of(31.067±0.11)mL·g^-1. Preliminary identification of extracted flavonoids showed flavones, flavonoids with adjacent two phenolic hydroxyl structures, and compounds with two phenol hydroxyl groups or 3-OH, 4=O or 5-OH, 4=O, and possible flavonoils, dihydroflavones and dihydro flavonols, but no chalcone and aurones. |
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| Keywords: | box-behnken response surface interaction effects Zelkova schneideriana flavonoids |
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