| 霍霍巴组培快繁技术体系研究 I.基本培养基与培养条件的优化 |
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| 引用本文： | 徐进,王玉珍,罗景兰,刘小京.霍霍巴组培快繁技术体系研究 I.基本培养基与培养条件的优化[J].河南农业科学,2004(8):21-24. |
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| 作者姓名： | 徐进 王玉珍 罗景兰 刘小京 |
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| 作者单位： | 1. 中国科学院遗传与发育生物学研究所农业资源研究中心,河北,石家庄,050021;中国科学院研究生院
2. 中国科学院遗传与发育生物学研究所农业资源研究中心,河北,石家庄,050021 |
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| 基金项目： | 河北省国际科技合作计划项目资助(03390162D) |
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| 摘 要： | 以霍霍巴为材料 ,研究了外植体的消毒、基本培养基、取材部位和培养条件等因素对组培苗生长的影响。结果表明 :采用 0 .1 %HgCl2 与 2 %NaClO 1∶1混合的消毒合剂处理外植体 8min ,灭菌成功率 99% ;改良MS培养基适合霍霍巴的分化培养 ;最适外植体为双腋芽茎段 ;培养条件 :温度 2 5～ 2 8℃ ,光照强度 30 0 0lx ,光照时间 1 2～ 1 4h/d ,pH 5 .5～ 6.0。
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| 关 键 词： | 霍霍巴 基本培养基 培养条件 |
| 文章编号： | 1004-3268(2004)08-0021-04 |
| 修稿时间： | 2004年3月9日 |
Studies on the Technique System of Tissue Culture and Rapid Propagation of Jojoba I.Selection of Basic Medium and Culture Condition |
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| XU Jin ,WANG Yu zhen ,LUO Jing lan ,LIU Xiao jing.Studies on the Technique System of Tissue Culture and Rapid Propagation of Jojoba I.Selection of Basic Medium and Culture Condition[J].Journal of Henan Agricultural Sciences,2004(8):21-24. |
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| Authors: | XU Jin WANG Yu zhen LUO Jing lan LIU Xiao jing |
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| Institution: | XU Jin 1,2,WANG Yu zhen 1,LUO Jing lan 1,LIU Xiao jing 1 |
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| Abstract: | The key techniques including explants disinfection, basic medium, suitable explant and culture condition were studied.By culturing the stems of Jojoba in vitro,the results showed as that the success percentage of disinfection reached 99% when the explants were treated by 1∶1 mixture of 0.1% HgCl 2 and 2% NaClO for eight minutes,the modified MS was suitable for Jojoba micropropagation.The optimal explants were stems with double axillary buds.All the cultures were kept under the temperature of 25-28 ℃,light intensity 3000 lx,light time for 12-14 h/d,and pH 5.5-6.0. |
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| Keywords: | Jojoba Basic medium Culture conditions |
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