| 旋毛虫期特异性基因的筛选与鉴定 |
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| 引用本文： | 姚春雨,汪明.旋毛虫期特异性基因的筛选与鉴定[J].中国畜牧兽医,2007,34(10):68-70. |
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| 作者姓名： | 姚春雨 汪明 |
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| 作者单位： | 1中国农业大学动物医学院， 北京 100094； 2内蒙古民族大学动物科技学院，通辽 028000 |
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| 摘 要： | 对旋毛虫新生幼虫 期特异性基因进行筛选与鉴定,结果表明:对新生幼虫cDNA文库进行核酸杂交筛选，获得7个阳性克隆，其大小在1.4 kb左右；用放射性同位素α32P标记cDNA探针后，与旋毛虫成虫、肌幼虫、新生幼虫、成虫/新生幼虫cDNA文库质粒DNA进行Southern印迹杂交，结果与成虫/新生幼虫、新生幼虫cDNA文库质粒DNA有杂交而与成虫、肌幼虫cDNA文库质粒DNA不杂交，因此该探针是新生幼虫期所特有的cDNA片段。
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| 关 键 词： | 旋毛虫 期特异性基因 筛选 鉴定 |
| 文章编号： | 1671-7236（2007）10-0068-02 |
| 修稿时间： | 2007-08-07 |
Bolting and Characterization of Stage Specificity Gene of Trichinella Spiralis |
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| YAO Chun-yu,WANG Ming.Bolting and Characterization of Stage Specificity Gene of Trichinella Spiralis[J].China Animal Husbandry & Veterinary Medicine,2007,34(10):68-70. |
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| Authors: | YAO Chun-yu WANG Ming |
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| Institution: | 1College of Veterinary Medicine，China Agricultural University，Beijing 100094，China；2Animal Science and Technology College, Inner Mongolla University For Nationality, Tongliao 028000，China |
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| Abstract: | Bolting and characterizing the gene of specificity gene of trichinella spiralis neogenesis larva, results indicate , getting seven positive clone by screening by hybridization of nucleic acid for cDNA Lib of neogenesis larva, about 1.4kb,after marking cDNA bougie by isotope a-3z P, Southern blotting hybridizating them with cDNA Lib plasmid DNA of trichinella spiralis neogenesis larva, imago, myo-larva, results indicate ,having hybridization with cDNA Lib plasmid DNA of trichina larva, imago,not of imago, myo-larva. Accordingly,this bougie is characteristic fragment of neogenesis larval phase. |
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| Keywords: | trichinella spiralis stage specificity gene bolting characteriz eation |
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