| 3种提取基因组DNA方法的比较分析 |
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| 引用本文： | 刘燕,刘孟洲,徐彦祥.3种提取基因组DNA方法的比较分析[J].中国畜牧兽医,2008,35(4):45-47. |
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| 作者姓名： | 刘燕 刘孟洲 徐彦祥 |
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| 作者单位： | 1.甘肃农业大学动物科技学院,兰州 730070；2.兰州猪场，榆中 730100 |
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| 摘 要： | 分别采用3种不同的方法提取基因组DNA，比较所提取的DNA的质量以及提取所需时间、费用，以期选择一种高效、简洁快速、价格合理的提取方法。结果发现这3种方法提取的质量都能达到相关分子生物学试验的要求，但在操作步骤、时间、价格等方面存在差异，具体比较结果可为分子生物学试验选择提取方法提供依据。
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| 关 键 词： | DNA提取 血液 组织 FTA采样卡 |
| 文章编号： | 1671-7236(2008)04-0045-03 |
| 修稿时间： | 2007年10月17 |
Comparison of Three Methods for Genomic DNA Extraction |
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| LIU Yan,LIU Meng-zhou,XU Yan-xiang.Comparison of Three Methods for Genomic DNA Extraction[J].China Animal Husbandry & Veterinary Medicine,2008,35(4):45-47. |
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| Authors: | LIU Yan LIU Meng-zhou XU Yan-xiang |
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| Institution: | 1.Faculty of Animal Science & Technology,Gansu Agriculture University,Lanzhou 730070,China;2.Lanzhou Swine Factory,Yuzhong 730100,China |
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| Abstract: | We separately used several different method to extracted genomic DNA,compared with several methods as well as the time,the expense and the time to choose an efficient,simple fast,reasonably priced extraction methods.Finally: Several methods could achieve the quality of molecular biology experiments related requirements,but in the sequence of operation,the time and the price there exist differences.Specific results of this comparison to be possible to provide the basis for the molecular biology experiment selective extraction method. |
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| Keywords: | DNA extraction blood organizations FTA sampling card |
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