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| 利用mtDNA控制区序列分析斑点叉尾(鮰)的遗传多样性 | |
| 引用本文： | 葛陇利,赫崇波,高祥刚,陈姝君,丛林林,曹洁.利用mtDNA控制区序列分析斑点叉尾(鮰)的遗传多样性[J].水产科学,2007,26(10):547-550. |
| 作者姓名： | 葛陇利 赫崇波 高祥刚 陈姝君 丛林林 曹洁 |
| 作者单位： | 1. 大连水产学院,生命科学与技术学院,辽宁,大连,116023 2. 辽宁省海洋水产科学研究院,辽宁省海洋水产分子生物学重点实验室,辽宁,大连,116023 3. 辽宁师范大学,生命学院,辽宁,大连,116029 |
| 摘 要： | 利用m tDNA控制区序列对1984、1997年引进的斑点叉尾鮰群体遗传多态性进行了分析。通过PCR产物测序法,测定了40尾斑点叉尾鮰线粒体DNA的一段包括部分Cytb基因、tRNAThr、tRNAPro、D-loop控制区和部分tRNAThe长度为1256 bp的核苷酸序列,经过比对分析得到7个序列单元型。7个单元型中共有13个碱基转换位点,全部发生在控制区,其序列相似度平均为99.58%。群体总的序列单元型多态性比例为17.5%,其中1997年群体的单元型多态性比例为35%,是1984年群体单元型多态性比例(15.0%)的2倍多。初步表明1984年引进的群体遗传多样性较低。 |
| 关 键 词： | 斑点叉尾(鮰) 线粒体DNA 单元型 遗传多样性 |
| 文章编号： | 1003-1111（2007）10-0547-04 |
| 修稿时间： | 2007-01-11 |
Analysis of Genetic Diversity of Channel Catfish (Ictalurus punctatus) by mtDNA Sequence |
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| GE Long-li,HE Chong-bo,GAO Xiang-gang,CHEN Shu-jun,CONG Lin-lin,CAO Jie.Analysis of Genetic Diversity of Channel Catfish (Ictalurus punctatus) by mtDNA Sequence[J].Fisheries Science,2007,26(10):547-550. | |
| Authors: | GE Long-li HE Chong-bo GAO Xiang-gang CHEN Shu-jun CONG Lin-lin CAO Jie |
| Institution: | 1.College of Life Science and Technology,Dalian Fisheries University,Dalian 116023,China;2.Liaoning Ocean and Fisheries Science Research Institute,Liaoning Key Lab of Molecular Biology of Marine Fisheries,Liaoning Open Lab of Applied Marine Biotechnology,Dalian 116023,China;3.College of Life Science,Liaoning Normal University,Dalian 116029,China |
| Abstract: | The genetic diversity of two populations of channel catfish(Ictalurus punctatus)(introduced in 1984 and 1999 from the USA) was analyzed using mtDNA control region.The sequences of partial Cytb,tRNAThr,tRNAPro,D-loop control region and partial tRNAThe were sequenced using PCR-based sequencing method from 40 individuals consisting of 1256 bp.Seven haplotypes were obtained from these 40 sequences whose similarity was 99.58% and there were 13 variation sites of transition in the control region and no variation in other genes.Percentage of haplotypes was 17.5% in the overall population,35% in the population 1997,and 15.0% in the population 1984,indicating that the genetic diversity of population 1984 was declining. |
| Keywords: | channel catfish(Ictalurus punctatus) mtDNA haplotype genetic diversity |
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