| 小美石斛茎段培养的研究 |
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| 引用本文： | 林泋君.小美石斛茎段培养的研究[J].中国农学通报,2006,22(2):292-292. |
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| 作者姓名： | 林泋君 |
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| 作者单位： | 云南农业职业技术学院,昆明,650031 |
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| 基金项目： | 云南农业职业技术学院校科研和教改项目 |
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| 摘 要： | 用小美石斛茎段作外殖体，进行试管繁殖。筛选出各培养阶段适宜的培养基，(1)腋芽萌生MS + 6-BA 5mg/L+NAA 0.4 mg/L；(2)继代增殖 MS + 6-BA 2mg/L+NAA0.2mg/L，三种培养基MS，1/2MS，N6，以MS增殖效果最好，(3)适宜的生根培养基为1/2MS + NAA0.1mg/L+PP333 0.1mg/L 其中平放的芽块生根速度，生根质量和生根率均高于直立放置的。
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| 关 键 词： | 小美石斛 茎段培养 |
| 收稿时间： | 2005-09-29 |
| 修稿时间： | 2005-09-292005-10-13 |
Stem-segment Culture and Tube Propagation of Dendrobium bellatulum Rolfe |
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| Lin Huijun.Stem-segment Culture and Tube Propagation of Dendrobium bellatulum Rolfe[J].Chinese Agricultural Science Bulletin,2006,22(2):292-292. |
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| Authors: | Lin Huijun |
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| Institution: | Y unnan Agriculture Vocational technical college, Kunming 650031 |
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| Abstract: | The stem-segment with axillary buds of Dendrobium bellatulum Rolfe as the explants, were cultured in tubes. The results of experiments suggested that appropriate media for respective culture stages were: (1) The germinaton of axillary buds: Ms 6-BA5mg/L NAA0.4mg/L (2) The differentiation and subculture Ms 6-BA2 mg/L NAA0.2 mg/L (3)Rootage: 1/2 MS NAA0.2mg/L PP333 was optional basal medium for proliferation , the rooting speed, number and percentage of adventitionous buds placed the medium levelly were significantly higher than thouse placed uprightly. |
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| Keywords: | Dendrobium bellatulum Rolfe Stem-segment culture Tube propagation |
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