北海道黄杨叶片应答低温胁迫的蛋白质组分析

 采用双向电泳的蛋白质组学方法分析了北海道黄杨叶片蛋白质对低温胁迫的应答反应。北海道黄杨植株在室外种植，从2008年10月—2009年2月每月提取叶片总蛋白。在考马斯亮蓝染色的2-DE胶上发现36个表达量显著差异蛋白点，其中通过质谱鉴定出19个蛋白质，包括已知的和新鉴定的低温胁迫相关蛋白质。这些鉴定出的蛋白质涉及多个生理过程，如能量与代谢、调控、防御应答等，同时这些蛋白质的表达模式与生理生化变化模式相一致。

Proteomic Analysis of Cold Stress Responses in Euonymus japonicus Leaves

A proteome study based on 2-D gel electrophoresis was performed in order to analyse the cold-stress response of Euonymus japonicus‘Cuzhi’leaves. The plants were grown in the outdoor and total proteins were extracted from leaves monthly from Oct，2008 to Feb，2009. Our results demonstrated significant variations existed in 36 protein spots detected on CBB stained 2-DE gels，among which 19 proteins were identified by mass spectrometry，including well known and newly identified cold-responsive proteins. The identified proteins were involved in several processes，such as energy and metabolism，regulatory，defense response etc. The expression patterns of these proteins were consistent with physiological and biochemical changes.