| 制备性分段电泳纯化基因工程白细胞介素—2 |
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| 引用本文： | 赵建中,赵建军,王晓光,孙晓燕,郝崇.制备性分段电泳纯化基因工程白细胞介素—2[J].中国兽医学报,1992(1). |
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| 作者姓名： | 赵建中 赵建军 王晓光 孙晓燕 郝崇 |
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| 作者单位： | 兽医大学生物工程研究室
(赵建中，赵建军，王晓光，孙晓燕)，兽医大学生物工程研究室(郝崇) |
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| 摘 要： | 采用具有高分辨率特性的聚丙烯酰胺凝胶为载体,以电泳技术将工程菌株的各种蛋白成分精细分离,再用紫外检测手段对白细胞介素—2(IL—2)基因表达产物在凝胶上定位,通过准确地切割凝胶,提取其中的蛋白质,获得了高纯度(>95%)的基因工程IL—2产物.经用同位素掺入法测定,其对小鼠胸腺细胞有明显的促进增殖活性
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| 关 键 词： | 白细胞介素-2 电泳 纯化 |
Purifying the rIL-2 from Engineering Bacterium, E. coli, with a Sectional Preparative Electrophoresis |
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| Zhao Jianzhong et al.Purifying the rIL-2 from Engineering Bacterium, E. coli, with a Sectional Preparative Electrophoresis[J].Chinese Journal of Veterinary Science,1992(1). |
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| Authors: | Zhao Jianzhong |
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| Abstract: | In this study, a column of polyacrylamide gel electrophoresis was used to isolate the various protein components from E. coli. After the proteins were located with UV -spec-trophotometer, the gel column was cut and 9 sections were obtained. Extracts from these sections were analysed with PAGE. It was found that the rIL-2 was located in the 2nd section of the gel. The identifying results showed that the purity of rIL- 2 is more than 95% and it has an obvious activity to facilitate the growth of mouse thymus cells. |
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| Keywords: | purification IL - 2 5 electrophoresis |
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