家蚕正反交组合F1代的基因表达系列分析(SAGE)文库构建

家蚕杂交组合存在普遍的正反交遗传表型差异现象,为探讨引起正反交组合遗传差异的分子机制,构建家蚕品种75新×7532正交F1代雌雄个体和7532×75新反交F1代雌雄个体共4个基因表达系列分析(SAGE)文库。通过正反交组合同性别子代样本间的比较,显示低表达的标签在种类上占大多数,而高表达的标签在数量上占绝对优势。以错误检测率(FDR)≤0.001且拷贝数倍数差异在2倍以上作为比较样本筛选差异表达基因(DEGs)的条件,在7532×75新的雄性样本里面有298个上调基因和46个下调基因,而在雌性样本里有453个上调基因和240个下调基因。进一步对差异表达基因进行GO和KEGG路径功能分析,筛选出在显著性富集路径中的差异表达基因,其中参与新陈代谢途径、氧化磷酸化途径以及信号传导途径的差异基因在反交组合的子代中表达明显上调。

Construction of Serial Analysis of Gene Expression (SAGE) Library of Reciprocal Cross Combination F1 Progeny in the Silkworm, Bombyx mori

Phenotypic difference is universal between reciprocal crosses in silkworm hybridized combinations.In order to explore the molecular mechanism causing genetic difference between reciprocal cross combinations,4 serial analysis of gene expression(SAGE) libraries,namely female and male individuals from silkworm F1 hybrids of 75xin×7532 and reverse cross 7532×75xin respectively,were constructed.A comparison on the samples from same sex progeny of reciprocal cross combinations showed that lowly expressed tags were dominant in kinds,while the highly expressed tags were superior in numbers.False discovery rate(FDR)≤0.001 and multiple difference of copy number>2 were set as the threshold to screen differentially expressed genes(DEGs) in compared samples.There were 298 up-regulated and 46 down-regulated genes in male individuals of 7532×75xin,and 453 up-regulated and 240 down-regulated genes in its female individuals.GO and KEGG pathway analyses were conducted to screen differentially expressed genes related to metabolic pathway,and the results showed that the DEGs involved in metabolic pathway,oxidative phosphorylation and signal transduction pathway were obviously up-regulated in the progeny of reverse cross combination.