药用植物灵菊七愈伤组织诱导及其染色体数目稳定性研究

研究了不同植物激素对药用植物灵菊七愈伤组织诱导、继代培养、细胞染色体稳定性的影响.结果表明,灵菊七的愈伤组织诱导、继代培养分别以MS+4.0 ng/L 6-BA+0.5 mg/L NAA、MS+2.0 mg/L 6-BA+0.5 mg/L NAA为培养基较适宜.培养在MS+2.0 mg/L 6-BA+0.5 mg/L NAA上的愈伤组织细胞中期分裂相染色体数目稳定,为20条,而培养在MS+2.0 mg/L ZT+0.5 mg/L NAA上的愈伤组织细胞中期分裂相染色体数目不稳定,其中86.7％的细胞含有20条染色体,13.3％的细胞含有10条染色体,表明激素ZT会导致愈伤组织细胞的染色体数目不稳定.在81个被观察的愈伤组织细胞中,超过85％的细胞染色体数目为20,表明灵菊七的体细胞染色体数应为2n=20.

Study on callus induction of Gynura medica and the stability of chromosome number in callus cells

It was investigated that different plant hormones effected on callus induction,callus regeneration,and stability of chromosome number in callus cells of Gynura medica.The result showed that medium MS+4.0 mg/L 6-BA+0.5 mg/L NAA,MS+2.0 mg/L 6-BA+0.5 mg/L NAA was fit for callus induction and callus regeneration respectively.Chromosomal number were stable,with 20 chromosomes,when callus was cultured on medium MS+2.0 mg/L 6-BA+0.5 mg/L NAA,while chromosomal number were unstable,when callus was cultured on the medium of MS+2.0 mg/L ZT+0.5 mg/L NAA,and showed 86.7% cells contained 20 chromosomes,13.3% cells contained 10 chromosomes.That meaned ZT enhanced chromosomes more unstable.In 81 cells,more than 85% callus cells contained stable 20 chromosomes,it was concluded that chromosome number of somatic cell of Gynura medica should be 2n=20.