金心扶芳藤组培快繁技术研究

为加快金心扶芳藤在园林绿化中的应用,以其带腋芽的茎段为材料,筛选其初代、增殖及生根培养基,建立组培快繁技术体系。结果表明:金心扶芳藤较适宜的初代诱导培养基为MS+BA 1.0 mg/L+IBA 0.05mg/L,芽的诱导率达96.0%;较适宜的增殖培养基为MS+BA 5.0 mg/L+IBA 0.08 mg/L+NAA 0.06 mg/L,芽的增殖系数达3.9;较适宜的生根培养基为1/2MS+IBA 2.0 mg/L,苗木生根率达100%,平均根数7.8条,根长4.5 cm。

Research on tissue culture and rapid propagation of‘Euonymus fortunei cv.Sunspot’

To speed up the application of‘Euonymus fortunei cv. Sunspot’in the landscape,with its stem segments with axillary buds of material,the initial screening,to establish tissue culture and rapid propagation technology system. Re-sults showed that,the appropriate medium on original culture was MS+BA 1.0 mg/L+IBA 0.05 mg/L,germination fre-quency of axillary buds reached 96.0%;The suitable proliferation medium were MS+BA5.0 mg/L+IBA0.08 mg/L+NAA 0.06 mg/L,and the multiplication coefficient were 3.9;The proper rooting medium was 1/2MS+IBA2.0 mg/L, rooting rate was up to 100%,root number 7.8,root length was 4.5 cm.