| 茶枝柑皮中多糖组成成分的分析 |
| |
| 引用本文： | 陈思,黄庆华,游明霞,吴韶辉,李娆玲,甘伟发.茶枝柑皮中多糖组成成分的分析[J].安徽农业科学,2011,39(32):19731-19733,19754. |
| |
| 作者姓名： | 陈思 黄庆华 游明霞 吴韶辉 李娆玲 甘伟发 |
| |
| 作者单位： | 广东药学院,广东广州510006;健康元药业集团股份有限公司;广东深圳518057;广东药学院,广东广州,510006;广东汤臣倍健生物科技股份有限公司,广东珠海,519040 |
| |
| 基金项目： | 珠海市科技工贸和信息化局企业技术创新项目(珠经贸字(2009)414号);广东省教育厅大学生创新实验项目资金资助(1057310008) |
| |
| 摘 要： | 目的]研究测定茶枝柑皮中多糖的组成成分。方法]以1-苯基-3-甲基-5-吡唑啉酮（PMP）进行柱前衍生化,采用反相高效液相色谱法,测定茶枝柑皮多糖中单糖衍生物。色谱条件为：色谱柱为Phenomenex Luna C18（4.6 mm×250 mm,5μm）;流动相为浓度0.05mol/L混合磷酸盐溶液-乙腈（80∶20,V/V,pH=7.1）;检测波长为245 nm。结果]茶枝柑皮多糖由甘露糖、核糖、鼠李糖、半乳糖醛酸、葡萄糖、半乳糖、木糖、阿拉伯糖组成,其摩尔比为0.08∶0.002∶0.05∶4.80∶1∶1.52∶0.11∶3.82。结论]该方法具有操作简单、快速、重复性好、回收率高等特点,可用于柑橘果胶多糖中单糖组成及含量的测定。
|
| 关 键 词： | 茶枝柑皮多糖 单糖 PR-HPLC 柱前衍生化 |
Analysis of Polysaccharides Composition of Citrus reticulata ' Chachi' Peel |
| |
| Institution: | CHEN Si et al(Guangdong？Pharmaceutical University,Guangzhou,Guangdong 51006) |
| |
| Abstract: | Objective] Polysaccharides composition of Citrus reticulata 'Chachi' peel was studied.Method] Taking PMP for pre-column derivatization,monosaccharide derivatives in polysaccharides of Citrus reticulata 'Chachi' peel were detected by Reversed-Phase High Performance Liqnid Chromatography(RP-HPLC).Chromatographic conditions were Phenomenex Luna C18(4.6 mm×250 mm,5 um),0.05 mol/L phosphate solution – acetonitrile(80∶20,V/V,pH=7.1) as mobile phase,and detection wavelength at 245 nm.Result] Polysaccharides of Citrus reticulata 'Chachi' peel were composed of mannose,ribose,rhamnose,galacturonic acid,glucose,galactose,xylose,and arabinose.Their molar ratio was 0.08:0.002:0.05:4.80:1:1.52:0.11:3.82.Conclusion] This method was simple,rapid,repeatable and has high recovery rate.Thus,it could be used for the determination of monosaccharide composition and content in citrus pectin polysaccharides. |
| |
| Keywords: | Citrus reticulata 'Chachi' peel polysaccharides Monosaccharide RP-HPLC pre-column derivatization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
