| 忍冬不同器官中绿原酸的提取及含量测定 |
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| 引用本文： | 刘宝臣,李瑞国,侯文锐. 忍冬不同器官中绿原酸的提取及含量测定[J]. 安徽农业科学, 2011, 39(32): 19734-19735,19774 |
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| 作者姓名： | 刘宝臣 李瑞国 侯文锐 |
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| 作者单位： | 泊头职业学院,河北泊头,062150;邢台学院生化系,河北邢台,054001;邢台市食品质量安全监督检验中心,河北邢台,054001 |
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| 基金项目： | 河北省邢台市科技计划项目“金银花绿原酸提取及含量研究”(201032071) |
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| 摘 要： | [目的]提取忍冬不同器官中的绿原酸并进行含量测定。[方法]采用酸乙醇回流法对＂巨花一号＂忍冬的藤、叶、花和花蕾中绿原酸进行提取,并采用高效液相色谱法对绿原酸进行检测和含量测定。[结果]忍冬不同器官中绿原酸的含量不同,其中花和花蕾中绿原酸的含量较高,藤和叶中含量较少;其排序为：花（3.798 mg/g）〉花蕾（3.795 mg/g）〉藤（2.558 mg/g）〉叶（2.185 mg/g）。[结论]从忍冬药材资源开发利用的角度而言,藤和叶也可以作为绿原酸提取和综合利用的材料。
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| 关 键 词： | 忍冬(Lonicera japonica Thunb) 绿原酸 提取 测定 |
Extraction and Content Determination of Chlorogenic Acid in Different Organs of Lonicera japonica Thunb |
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| Affiliation: | LIU Bao-chen et al(Botou College,Botou,Hebei 062150) |
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| Abstract: | [Objective] To extract the chlorogenic acid in different organs of Lonicera japonica Thunb.and then determine its content.[Method] Chlorogenic acid in the vine,leaves,flowers and buds of "Giant Flower One" honeysuckles was extracted by acid ethanol method,and then determined by HPLC.[Result] The content of chlorogenic acid was different in different organs of honeysuckles,it was higher in flowers and buds,while lower in vines and flowers;which was shown as flowers(3.798 mg/g)> buds(3.795 mg/g)> vines(2.558 mg/g)>leaves(2.185 mg/g).[Conclusion] The veins and leaves of Lonicera japonica Thunb.could be used as the materials for developing medical resources, |
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| Keywords: | Lonicera japonica Thunb. Chlorogenic acid Extraction Determination |
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