小麦花培突变体新品种川辐5号的选育及相关技术研究

用 1 50Gy的6 0 Coγ射线诱变处理 (绵阳 88 3 3 4× 88 1 1 52 5)杂交当代干种子 ;从MF2 代中选择优良单株为供试材料 ,以MW1 4和改良MS为基本培养基进行离体花药培养 ,获得MH1代纯合二倍体花粉株系 ;1 996年选育出遗传稳定、综合性状优良的花培突变体新品系 60 86及其姊妹系 60 85和 60 87。 2 0 0 2年新品系 60 86通过四川省农作物品种审定委员会审定 ,命名为川辐 5号。表明利用辐射诱变并结合花药培养 ,可有效地缩短小麦育种周期 ,提高辐射育种效率

THE BREEDING OF A WHEAT MUTANT POLLEN-DERIVED VARIETY CHUANFU No.5 AND THE RELATED TECHNIQUES

With the treatment of 150Gy 60 Co\|γ irradiation to the dry F\-1(Mianyang 88\|334×88\|11525) hybrid seeds and the donor plants chosen from MF\-2,wheat anther culture was made based on MW 14 and modified MS media and the pure diploid lines of MH\-1 derived from anther pollen were obtained.In 1996,the new mutant line 6086 and its sibling lines,6086 and 6087, were selected and bred successfully.In 2002,the mutant pollen\|derived line 6086 was denominated as Chuanfu No.5 by Sichuan Crop Variety Identification Committee and became the first mutant variety via anther culture of wheat in Sichuan.The success of Chuanfu No.5 shown that combining radiation induction and anther technique could shorten the breeding period and increase the efficiency of breeding of wheat.