黄芪提取物对常见致病菌的体内外抑菌活性测定

为了研究黄芪提取物对金黄色葡萄球菌、大肠杆菌和沙门氏菌等致病菌的抑菌活性,采用试管二倍稀释法,测定黄芪提取物对金黄色葡萄球菌、大肠杆菌和沙门氏菌的体外抑菌活性;采用相同浓度致病菌灌胃小鼠后,分别用黄芪提取物灌胃治疗,测定小鼠十二指肠、空肠、回肠和盲肠中肠道菌群数量,以确定黄芪提取物的体内抑菌活性.结果表明,无论是在体外还是在体内,黄芪提取物对金黄色葡萄球菌、大肠杆菌和沙门氏菌均具有抑制作用,对大肠杆菌的抑制作用最强,对沙门氏菌的抑制作用最弱;且通过灌注黄芪提取物,显著降低了各种致病菌致病后小鼠的死亡率.通过研究可以得出,黄芪提取物对常见的金黄色葡萄球菌、大肠杆菌和沙门氏菌等致病菌具有较明显的体内外抑菌作用.

Determination of Antibacterial Activity of Extract of Astragalus on Common Pathogenic Bacteria in vitro and in vivo

In order to study the antibacterial activity of Astragalus extract on Staphylococcus aureus ,Escherichia coli and Salmonella sp., the antibacterial activity of Astragalus extract on S. aureus, E. coli and Salmonella sp. in vitro was determined by using tube double dilution method. Mice were administrated with the same concentration of pathogenic bacteria by intragastric administration to treat with Astragalus extract. The number of intestinal bacteria in the duodenum,jejunum,ileum and cecum of mice were respectively determined so as to study the antibacterial activity of Astragalus extract in vivo. The results showed that Astragalus extract had inhibitory effects on S. aureus, E. coli and Salmonella sp. in vitro and in vivo. And Astragalus extract had strongest inhibition on E. coli and weakest inhibition on Salmonella sp. The mortality rate of mice after infecting with various kinds of pathogenic bacteria was significantly reduced by perfusing Astragalus extract. In conclusion, Astragalus extract had obvious antibacterial effects on S. aureus ,E. cali and Salmonella sp. in vitro and in vivo.