Haploidy breeding and mutagenesis for drought tolerance in wheat |
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Authors: | AJ Khan S Hassan M Tariq T Khan |
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Institution: | (1) Nuclear Institute for Food and Agriculture (NIFA), Tarnab-Peshawar, P.O. Box 446, Pakistan |
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Abstract: | Several intraspecific crosses between known drought tolerant wheat varieties and stable high yielding recombinants were made
with the objective to develop improved cultivars for the moisture stressed rainfed areas of Pakistan. Five of these crosses
were selected for further creation of useful mutations through the application of low doses of gamma rays and development
of doubled haploids through anther culture. Anther culture response of the selected irradiated F1 generations was studied on liquid and solid induction media. The highest number of calli among almost all crosses was produced
on Potato-2induction medium. All the crosses varied greatly in response to callus induction and maximum calli (75%) were obtained
from Lyl-73/vee’s’ cross. Similarly, genotypic differences were found for green vs. albino regenerants. The highest number
of green plantlets (12.1%) was recorded for Lu-26/3062. From the developed doubled haploid population 25 DH-mutants were initially
selected and nine lines were finally included in multi-locational field tests. Two DH-mutants (i.e. DHML-50 and DHML-9) have
potential for better grain yield, earliness, disease resistance and moisture stress tolerance.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | anther culture DH-mutants doubled haploids gamma rays mutagenesis mutants Triticum aestivum |
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