Genetic variation in mitochondrial genes and intergenic spacer region in harmful algae <Emphasis Type="Italic">Chattonella</Emphasis> species |
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Authors: | Ryoma Kamikawa Isao Masuda Kenichi Oyama Sadaaki Yoshimatsu Yoshihiko Sako |
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Institution: | (1) Laboratory of Marine Microbiology, Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, 606-8502 Kyoto, Japan;(2) Akashiwo Research Institute of Kagawa Prefecture, 75-5 Yashima-Higashi-machi, 761-0111 Takamatsu, Kagawa, Japan |
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Abstract: | In this study, nuclear ribosomal RNA gene internal transcribed spacer regions and the cox2-cox1 fragment of the mitochondrial (mt) genome were sequenced in 24 strains of Chattonella spp. Variability in both regions showed that the mt genome sequences of Chattonella spp. have a higher evolutionary rate than the nuclear rRNA gene sequences. A maximum likelihood tree based on the mt sequence
grouped the Japanese Chattonella strains into two groups (Groups A and B), although no correlation was observed amongst the phylogenetic groups, their morphologies,
or the isolated areas. Groups A and B were clearly identified by a polymerase chain reaction-restriction fragment length polymorphism
(PCR-RFLP) assay using Fokl, without the need for a sequencing experiment. The PCR-RFLP assay revealed that Chattonella cells obtained from sea water in Oita, Japan, in 2004 and 2005 belonged to Group B. This is the first report showing the
genetic variation in Chattonella spp. using a PCR-RFLP identification protocol. |
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Keywords: | Chattonella spp cytochrome c oxidase subunit 1 (cox1) cytochrome c oxidase subunit 2 (cox2) genetic diversity harmful algae mitochondria |
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