| 甘蓝型油菜渝黄1号黄籽性状的AFLP和SSR标记 |
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| 引用本文： | 梅德圣,李云昌,王汉中,李英德,徐育松,胡琼.甘蓝型油菜渝黄1号黄籽性状的AFLP和SSR标记[J].中国油料作物学报,2004,26(3):6-9. |
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| 作者姓名： | 梅德圣 李云昌 王汉中 李英德 徐育松 胡琼 |
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| 作者单位： | 中国农业科学院油料作物研究所,国家油料作物改良中心,农业部油料作物遗传改良重点实验室,湖北,武汉,430062 |
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| 基金项目： | 国家高技术研究发展计划(863计划)，引进国际先进农业科技计划(948计划)，农业部重点实验室基金 |
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| 摘 要： | 以甘蓝型黄籽杂交油菜渝黄1号为试验材料,用F2群体中的9株黑籽单株和12株黄籽单株的DNA分别构成黑籽集团和黄籽集团.通过BSA法筛选了96个AFLP引物组合和173对SSR引物,鉴定出与显性黄籽基因连锁的1个AFLP标记E35M52180和1个SSR标记P039230,标记与显性黄籽基因的交换率分别为4.9%和2.5%.此外还发现1个SSR标记P055240与深色种皮有关.
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| 关 键 词： | 甘蓝型油菜 显性黄籽基因 AFLP标记 SSR标记 |
| 文章编号： | 1007-9084(2004)03-0006-04 |
| 修稿时间： | 2004年4月26日 |
Identification of AFLP and SSR markers associated with yellow-seeded trait in Brassica napus Yuhuang No.1 |
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| MEI De-sheng,LI Yun-chang,WANG Han-zhong,LI Ying-de,XU Yu-song,HU Qiong.Identification of AFLP and SSR markers associated with yellow-seeded trait in Brassica napus Yuhuang No.1[J].Chinese Journal of Oil Crop Sciences,2004,26(3):6-9. |
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| Authors: | MEI De-sheng LI Yun-chang WANG Han-zhong LI Ying-de XU Yu-song HU Qiong |
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| Abstract: | DNA samples from 9 blacked-seeded and 12 yellow-seeded F_2 plants, which derived from Brassica napus variety Yuhuang No.1, were mixed respectively to make blacked-seeded bulk and yellow-seeded bulk. 96 AFLP primer combinations and 173 pairs of SSR primers were selected with the bulked segregant analysis (BSA) approach. One AFLP marker E35M52_(180) and one SSR marker P039_(230) were identified to be tightly linked to dominant yellow-seeded genes, with recombination value of 4.9% and 2.5% respectively. Another SSR marker P055_(240), which related to dark seed coat color, was found in this experiment. |
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| Keywords: | Brassica napus L Dominant yellow-seeded gene AFLP marker SSR marker |
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