Establishment of PCR-ELISA for Detecting Glyphosate Resistant Transgenic Soybean |
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| Institution: | 1. College of Life Sciences, Northeast Agricultural University, Harbin 150030, China;2. Heilongjiang Forest Protection Research Institute, Harbin 150040, China;3. Heilongjiang Institute of Education, Harbin 150080, China;4. Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China |
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| Abstract: | A PCR-ELISA method for detecting the glyphosate resistant transgenic soybean was established and optimized. The results showed that the key parameters of PCR-ELISA were as follows: the concentration of digoxin tag probe was 0.5 μmol? L-1, the time of hybridization reaction was 15 min and the chromogenic reaction should last for 30 min. The sensitivity and the repeatability of our PCR-ELISA method were evaluated, and the results showed that it could be detected when the concentration of DNA template from transgenic soybean samples was 0.01% or higher, and the coefficient of variation of this method was less than 5% in our research condition. These results suggested that PCR-ELISA method establishment in this study had good repeatability and high precision for detecting the transgenic soybean samples. |
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| Keywords: | PCR ELISA glyphosate resistant transgenic soybean |
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