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Optimization of chitosan flocculation for phytoplankton removal in shrimp culture ponds
Authors:Pranee Lertsutthiwong  Suttikarn Sutti  Sorawit Powtongsook
Institution:1. Metallurgy and Materials Science Research Institute, Chulalongkorn University, Bangkok 10330, Thailand;2. Center for Chitin-Chitosan Biomaterials, Chulalongkorn University, Bangkok 10330, Thailand;3. National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand;4. Center of Excellence for Marine Biotechnology, Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand;1. Laboratory for Aquatic Biology, KU Leuven Campus Kulak, Etienne Sabbelaan 53, 8500 Kortrijk, Belgium;2. Department of Biotechnology, School of Science, Kathmandu University, Dhulikhel, Nepal;3. KU Leuven Kulak, Research Unit Food & Lipids, Department of Molecular and Microbial Systems Kulak, Etienne Sabbelaan 53, B-8500 Kortrijk, Belgium;4. Leuven Food Science and Nutrition Research Centre (LFoRCe), KU Leuven, Kasteelpark Arenberg 20, B-3001 Heverlee, Belgium;5. bioMASS lab, School of Chemical Engineering, The University of New South Wales, Sydney 2052, Australia;1. Department of Civil and Environmental Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejon 305-701, Republic of Korea;2. Department of Environmental Sciences, COMSATS Institute of Information Technology, Abbottabad 22060, Pakistan;1. School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China;2. School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China;3. Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai, Nada-ku, Kobe 657-8501, Japan;4. Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan;5. University Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan 701, Taiwan;6. Research Center for Energy Technology and Strategy Center, National Cheng Kung University, Tainan 701, Taiwan;1. Department of Chemical Engineering, National Taiwan University of Science and Technology, 43 Keelung Road, Section 4, Taipei 106, Taiwan;2. Department of Chemical Engineering, Widya Mandala Catholic University, Kalijudan 37, Surabaya 60114, Indonesia
Abstract:The removal of phytoplankton cells from aquaculture systems generally results in the reduction of nitrogenous waste and improves water quality. With this study, the effects of chitosan concentration, environmental condition and pH adjustment on flocculation of phytoplankton in marine shrimp (Litopenaeus vannamei) culture tanks were investigated. The remaining phytoplankton and suspended solids in the system were indicators for evaluating the efficiency of chitosan on flocculation. The results indicate that the flocculation efficiency of chitosan was highest (>85%) and remained fairly constant at a chitosan concentration of 40–80 mg L?1 and a pH range of 7–9 after chitosan addition. With this novel technique including 40 mg L?1 chitosan addition, pH adjustment to 6.5 and then to 8.5, high efficiency and consistency of flocculation were achieved. This technique could also be applied with various water alkalinity up to 400 mg CaCO3 L?1. The experiment for phytoplankton removal by chitosan flocculation in the recirculating aquaculture system showed that flocculation efficiency remained constant even though flocculation was repeated several times.
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