| 梨矮化基因pcDw的一个SCAR标记 |
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| 引用本文： | 贾彦利,王彩虹,田义轲,戴洪义,王亮.梨矮化基因pcDw的一个SCAR标记[J].园艺学报,2007,34(6):1531-1534. |
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| 作者姓名： | 贾彦利 王彩虹 田义轲 戴洪义 王亮 |
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| 作者单位： | （青岛农业大学园艺学院，山东青岛 266109） |
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| 基金项目： | 山东省自然科学基金;山东省青岛市科技攻关项目;山东省农业良种产业化工程项目 |
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| 摘 要： | 以'矮化梨'(Pyrus communis L.)与 '茌梨'(Pyrus bretschneideri Rehd.)的杂交后代共111个单株为试材，采用分离群体分组分析法（Bulked Segregate Analysis, BSA），通过对412个随机引物的筛选，获得了一个与控制梨树矮化性状基因pcDw连锁距离为8.3 cM、长度为940 bp的RAPD标记S1172-940, 并将其转换成了SCAR (Sequence Characterized Amplified Region) 标记，即SCAR-940。这一研究结果，为该矮化性状的标记辅助选择提供了有效工具。
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| 关 键 词： | 梨 矮化 RAPD SCAR |
| 文章编号： | 0513-353X（2007）06-1531-04 |
| 收稿时间： | 2007-04-09 |
| 修稿时间： | 2007-09-07 |
A SCAR Marker Linked to pcDw Gene in Pear |
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| JIA Yan-li,WANG Cai-hong,TIAN Yi-ke,DAI Hong-yi,WANG Liang.A SCAR Marker Linked to pcDw Gene in Pear[J].Acta Horticulturae Sinica,2007,34(6):1531-1534. |
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| Authors: | JIA Yan-li WANG Cai-hong TIAN Yi-ke DAI Hong-yi WANG Liang |
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| Institution: | (College of Horticulture, Qingdao Agricultural University, Qingdao,Shandong 266109, China) |
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| Abstract: | To identify molecular markers linked to pcDw gene determining the dwarf character of pear tree, a population of 111 individuals from the cross of 'Aihuali'(Pyrus communis L.)×'Chili'(Pyrus bretschneideri Rehd.) was tested using 412 random primers by the way of bulked segregate analysis(BSA). A RAPD marker, S1172-940, linked to pcDw gene at a distance of 8.3 cM, was detected. This RAPD marker was converted into a SCAR (Sequence Characterized Amplified Region) marker, SCAR-940, which would provide an effective tool for marker assisted selection for the dwarf trait. |
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| Keywords: | RAPD SCAR |
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