| Analysis of LMW-GS, α- and γ-Gliadin Gene Coding Sequences from Triticum macha |
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| 作者姓名： | XIONG Li-juan WANG Ji-rui ZHENG You-liang |
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| 作者单位： | [1]Triticeae Research Institute, Sichuan Agricultural University, Ya＇an 625014, P.R.China [2]Key Laboratory of Southwestern Crop Germplasm Utilization, Ministry of Agriculture, Ya＇an 625014, P.R.China |
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| 基金项目： | 国家高技术研究发展计划(863计划)，the Key Technologies R&D Program |
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| 摘 要： | Novel LMW-GS （low molecular weight glutenin subunit）,α- and γ-gliadin from Triticum macha accessions were characterized via genomic PCR, which can do favor to improve the wheat quality. The complete coding regions of two α-gliadin, two γ-gliadin and two LMW-GS gene sequences, which designed as Gli-Mal, Gli-Ma2, Gli-Mrl, Gli-Mr2, Glu-LM1 and Glu-LM2, encoded the mature proteins with 307, 241, 348, 302, 474 and 377 amino acid residues, respectively. Gli-Mal and Gli-Ma2 were recognized as pseudogenes due to the in-frame stop codons. The amino acid sequences deduced from these gene sequences were characterized with the typical structure of α- or γ-gliadin or LMW- m type proteins with the exception of Gli-Ma2. Phylogenetic analysis showed Gli-Mal was closely related to those from T. aestivum, whereas Gli-Ma2 seemed to be more homologous with the gene sequences from Dasypyrum breviaristatum. Gli-Mrl was closely related to those from T. turgidum ssp. dicoccoides, while Gli-Mr2 was the nearest to those from T. aestivum. Glu-LM1 was closely related to those from Aegilops tauschii, whereas GIu-LM2 seemed to be more homologous with those from T. durum.
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| 关 键 词： | 低分子量谷蛋白亚基 醇溶蛋白基因 编码序列 小麦品质 基因序列 系统发育分析 普通小麦 麦醇溶蛋白 |
| 收稿时间： | 3 May 2009 |
Analysis of LMW-GS,α-and γ-Gliadin Gene Coding Sequences from Triticum macha |
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| XIONG Li-juan,WANG Ji-rui,ZHENG You-liang.Analysis of LMW-GS,α-and γ-Gliadin Gene Coding Sequences from Triticum macha[J].Agricultural Sciences in China,2010,9(2):163-169. |
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| Authors: | XIONG Li-juan WANG Ji-rui ZHENG You-liang |
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| Institution: | 1. Department of Plant Pathology, College of Plant Protection, Hebei Agricultural University, Baoding 071001, Hebei, China;2. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China;3. International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600 Mexico D.F., Mexico |
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| Abstract: | Novel LMW-GS(low molecular weight glutenin subunit),α-and γ-gliadin from Triticum macha accessions were characterized via genomic PCR,which can do favor to improve the wheat quality.The complete coding regions of two α-gliadin,two γ-gliadin and two LMW-GS gene sequences,which designed as Gli-Mal,Gli-Ma2,Gli-Mr1,Gli-Mr2,Glu-LM1 and Glu-LM2,encoded the mature proteins with 307,241,348,302,474 and 377 amino acid residues,respectively.Gli-Mal and Gli-Ma2 were recognized as pseudogenes due to the in-frame stop codons.The amino acid sequences deduced from these gene sequences were characterized with the typical structure of α-or γ-gliadin or LMW-m type proteins with the exception of Gli-Ma2.Phylogenetic analysis showed Gli-Mal was closely related to those from T.aestivum,whereas Gli-Ma2 seemed to be more homologous with the gene sequences from Dasypyrum breviaristatum.Gli-Mr1 was closely related to those from T.turgidum ssp.dicoccoides,while Gli-Mr2 was the nearest to those from T.aestivum.Glu-LM1 was closely related to those from Aegilops tauschii,whereas Glu-LM2 seemed to be more homologous with those from T.durum. |
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| Keywords: | T macha LMW-GS gene clone sequence analysis |
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