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Bulk tank milk ELISA for detection of antibodies to Mycobacterium avium subsp. paratuberculosis: Correlation between repeated tests and within-herd antibody-prevalence
Authors:Søren Saxmose Nielsen  Nils Toft
Institution:1. Department of Large Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegårdsvej 8, DK-1870 Frederiksberg C, Denmark;2. Technical University of Denmark, National Veterinary Institute, Bülowsvej 27, DK-1870 Frederiksberg C, Denmark
Abstract:Detection of bulk tank milk (BTM) antibodies using ELISA (BTM-ELISA) may constitute an inexpensive test for surveillance of Mycobacterium avium subsp. paratuberculosis (MAP) infection in dairy cattle herds provided that the test is accurate and consistent. The objectives of this study were to determine: (a) the correlation between repeated BTM reactions; and (b) the association between the BTM antibody ELISA-level and the within-herd prevalence of antibody-positive cows.
Keywords:Bulk tank milk ELISA  Cattle  Paratuberculosis  Herd diagnosis
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