植物生长调节剂对甜椒的抗寒性诱导效应研究

以5个品种的甜椒为试材,研究了由冠菌素(COR)、脱落酸(ABA)和矿源黄腐酸(MFA)3种调节剂复配而成的植物生长调节剂MIXⅡ对提高甜椒抗寒性的诱导效应。幼苗移植7 d后,用三元复配剂MIXⅡ进行灌根处理(10 mL/株),在10~15 ℃下生长60 d后,在低温(5 ℃,48 h)胁迫下,分析各品种甜椒幼苗生长、细胞质膜透性、渗透调节物质、植株体内过氧化氢及其抗氧化酶活性与各自对照之间的差异。结果表明:MIXⅡ在低温逆境下对不同品种甜椒幼苗生长具有不同的保护效应;在代谢水平上可提高不同甜椒品种的相关抗氧化酶活性,减轻活性氧对细胞质膜的伤害,降低相对电导率;同时可提高低温环境下叶片脯氨酸、K+等渗透调节物质的含量,保持细胞的渗透势,维持细胞正常的代谢功能,从而提高不同品种甜椒的抗寒性。

Inducing effects of plant growth regulators on the chilling resistance of sweet pepper (Capsicum annuum)

The inducing effects of plant growth regulator MIX Ⅱ,which is the mixture of coronatine (COR),abscisic acid (ABA) and mineral fulvic acid (MFA),was analyzed on increasing the chilling resistance of five sweet pepper varities.After transplanting for 7 d,seedling roots were watered by 10 mL MIXⅡ per plant,then seedlings were grew under 10-15 ℃ for 60 d,and then were exposed to low temperature (5 ℃) for 48 h.The seedling growth, cytoplasmic membrane permeability,osmoregulation substance,the hydrogen peroxide and antioxidant enzyme activity were measured in different sweet pepper varieties, and the differences of these characteristics were analyzed between the treatments and control.The results showed that the protection effects of MIXⅡon the seedlings growth of different sweet pepper varieties were different.The antioxidant enzymes activities were increased,the injury of oxygen to cytoplasmic membrane and the relative electrolyte leakage were reduced;The content of osmoregulation substance of proline and K+ in seedling leaves were increased,and the cell osmotic potential and the cell metabolism were maintained at normal level.As a result,the chilling resistance of different sweet pepper varieties were improved by MIXⅡunder chilling stress.